College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
Molecules. 2021 May 10;26(9):2803. doi: 10.3390/molecules26092803.
Pancreatic cancer is one of the most malignant cancers with high mortality. Therefore, it is of great urgency to develop new agents that could improve the prognosis of Pancreatic cancer patients. Chinese propolis (CP), a flavonoid-rich beehive product, has been reported to have an anticancer effect. In this study, we applied CP to the human Pancreatic cancer cell line Panc-1 to verify its impact on tumor development. CP induced apoptosis in Panc-1 cells from 12.5 µg/mL in a time- and dose-dependent manner with an IC value of approximately 50 µg/mL. Apoptosis rate induced by CP was examined by Annexing FITC/PI assay. We found that 48 h treatment with 50 µg/mL CP resulted in 34.25 ± 3.81% apoptotic cells, as compared to 9.13 ± 1.76% in the control group. We further discovered that the Panc-1 cells tended to be arrested at G2/M phase after CP treatment, which is considered to contribute to the anti-proliferation effect of CP. Furthermore, our results demonstrated that CP suppressed Panc-1 cell migration by regulating epithelial-mesenchymal transition (EMT). Interestingly, the Hippo pathway was activated in Panc-1 cells after CP treatment, serving as a mechanism for the anti-pancreatic cancer effect of CP. These findings provide a possibility of beehive products as an alternative treatment for pancreatic cancer.
胰腺癌是一种死亡率极高的恶性肿瘤。因此，开发新的药物来改善胰腺癌患者的预后迫在眉睫。中国蜂胶（CP）是一种富含类黄酮的蜂巢产品，已被报道具有抗癌作用。在这项研究中，我们将 CP 应用于人类胰腺癌细胞系 Panc-1 中，以验证其对肿瘤发展的影响。CP 以时间和剂量依赖的方式诱导 Panc-1 细胞凋亡，IC 值约为 50μg/ml。通过 Annexin FITC/PI 检测法检测 CP 诱导的细胞凋亡率。我们发现，与对照组的 9.13±1.76%相比，50μg/ml CP 处理 48 小时后，凋亡细胞比例为 34.25±3.81%。我们进一步发现，CP 处理后 Panc-1 细胞倾向于停滞在 G2/M 期，这被认为是 CP 抗增殖作用的原因。此外，我们的结果表明 CP 通过调节上皮-间充质转化（EMT）抑制 Panc-1 细胞迁移。有趣的是，CP 处理后 Hippo 通路在 Panc-1 细胞中被激活，这是 CP 发挥抗胰腺癌作用的机制之一。这些发现为蜂巢产品作为胰腺癌的替代治疗提供了可能性。
