NudC 介导的 dpCoA-RNA 脱帽的结构见解。
Structural insights into dpCoA-RNA decapping by NudC.
机构信息
National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.
State Key Laboratory of Hybid Rice, College of Life Sciences, Wuhan University, Wuhan, Hubei, China.
出版信息
RNA Biol. 2021 Oct 15;18(sup1):244-253. doi: 10.1080/15476286.2021.1936837. Epub 2021 Jun 18.
Various kinds of cap structures, such as mG, triphosphate groups, NAD and dpCoA, protect the 5' terminus of RNA. The cap structures bond covalently to RNA and affect its stability, translation, and transport. The removal of the caps is mainly executed by Nudix hydrolase family proteins, including Dcp2, RppH and NudC. Numerous efforts have been made to elucidate the mechanism underlying the removal of mG, triphosphate group, and NAD caps. In contrast, few studies related to the cleavage of the RNA dpCoA cap have been conducted. Here, we report the hydrolytic activity of NudC towards dpCoA and dpCoA-capped RNA . We also determined the crystal structure of dimeric NudC in complex with dpCoA at 2.0 Å resolution. Structural analysis revealed that dpCoA is recognized and hydrolysed in a manner similar to NAD. In addition, NudC may also remove other dinucleotide derivative caps of RNA, which comprise the AMP moieties. NudC homologs in and exhibited similar dpCoA decapping (deCoAping) activity. These results together indicate a conserved mechanism underpinning the hydrolysis of dpCoA-capped RNA in both prokaryotes and eukaryotes.
各种帽结构，如 mG、三磷酸基团、NAD 和 dpCoA，保护 RNA 的 5'末端。帽结构与 RNA 共价结合，影响其稳定性、翻译和运输。帽的去除主要由 Nudix 水解酶家族蛋白执行，包括 Dcp2、RppH 和 NudC。人们已经做出了许多努力来阐明去除 mG、三磷酸基团和 NAD 帽的机制。相比之下，关于 RNA dpCoA 帽的切割的研究很少。在这里，我们报告了 NudC 对 dpCoA 和 dpCoA 帽 RNA 的水解活性。我们还确定了 2.0 Å 分辨率下二聚体 NudC 与 dpCoA 复合物的晶体结构。结构分析表明，dpCoA 以类似于 NAD 的方式被识别和水解。此外，NudC 可能还会去除 RNA 的其他二核苷酸衍生物帽，其中包含 AMP 部分。和 中的 NudC 同源物表现出相似的 dpCoA 脱帽（deCoAping）活性。这些结果共同表明，在原核生物和真核生物中，dpCoA 帽 RNA 的水解存在保守机制。
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