快速眼动睡眠剥夺和睡眠恢复对循环神经炎症标志物的影响。
Impact of REM sleep deprivation and sleep recovery on circulatory neuroinflammatory markers.
作者信息
Suresh Konakanchi, Shankar Vinutha, Cd Dayanand
机构信息
Sri Devaraj Urs Medical College, Sri Devaraj Urs Academy of Higher Education and Research, Department of Physiology - Kolar - Karnataka - India.
Sri Devaraj Urs Medical College, Sri Devaraj Urs Academy of Higher Education and Research, Department of Biochemistry - Kolar - Karnataka - India.
出版信息
Sleep Sci. 2021 Jan-Mar;14(1):64-68. doi: 10.5935/1984-0063.20190157.
OBJECTIVES
Sleep loss may contribute to neuroinflammation, which might increase neuroinflammatory markers such as neuron-specific enolase (NSE), creatine kinase-brain fraction (CK-BB), lactate dehydrogenase brain fraction (LDH-BB) in blood. Hence, we evaluated the effect of REM sleep deprivation and recovery on these markers.
MATERIAL AND METHODS
Twenty-four adult male Sprague Dawley rats were grouped as control, environmental control, REM sleep deprivation, and 24 hour sleep recovery. The rats were sleep deprived for 72 hours and recovered for 24 hours. NSE, CK-BB, and LDH-BB levels in serum were measured using ELISA.
RESULTS
The serum NSE, CK-BB, and LDH-BB were significantly higher in 72 hour sleep deprived group compared to control (<0.01). After 24 hours of sleep recovery, the levels of NSE, CK-BB, and LDH-BB were comparable to control (>0.05).
DISCUSSION
REM sleep deprivation increased serum NSE, CK-BB, and LDH-BB, which might be due to neural damage. However, 24 hours of sleep recovery restored these markers.
目的
睡眠不足可能导致神经炎症，这可能会增加血液中神经元特异性烯醇化酶（NSE）、脑型肌酸激酶（CK-BB）、脑型乳酸脱氢酶（LDH-BB）等神经炎症标志物的水平。因此，我们评估了快速眼动睡眠剥夺及恢复对这些标志物的影响。
材料与方法
将24只成年雄性Sprague Dawley大鼠分为对照组、环境对照组、快速眼动睡眠剥夺组和24小时睡眠恢复组。大鼠被剥夺睡眠72小时，然后恢复24小时。采用酶联免疫吸附测定法（ELISA）检测血清中NSE、CK-BB和LDH-BB的水平。
结果
与对照组相比，72小时睡眠剥夺组血清中的NSE、CK-BB和LDH-BB显著升高（<0.01）。睡眠恢复24小时后，NSE、CK-BB和LDH-BB的水平与对照组相当（>0.05）。
讨论
快速眼动睡眠剥夺会使血清中的NSE、CK-BB和LDH-BB升高，这可能是由于神经损伤所致。然而，24小时的睡眠恢复使这些标志物恢复正常。
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