追风透骨胶囊通过调节 Toll 样受体 2/4-核因子 kappa-B 信号通路改善类风湿关节炎大鼠的症状。

Zhuifeng Tougu capsules improve rheumatoid arthritis symptoms in rats by regulating the toll-like receptor 2/4-nuclear factor kappa-B signaling pathway.

机构信息

Department of oncology, Hunan university of Chinese medicine integrated Chinese and Western Affiliated Hospital, Changsha 410007, China.

Department of orthopedics, the First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007, China.

出版信息

J Tradit Chin Med. 2021 Jun;41(3):447-454. doi: 10.19852/j.cnki.jtcm.2021.03.013.

DOI:10.19852/j.cnki.jtcm.2021.03.013

PMID:34114403

Abstract

OBJECTIVE

To investigate the efficacy of Zhuifeng tougu capsules (, ZFTG) in the treatment of rheumatoid arthritis (RA) in rats and study its mechanism, focusing on the toll-like receptor 2/4-nuclear factor kappa-B (TLR2/4-NF-κB) signaling pathway.

METHODS

Type Ⅱ collagen and an artificial climate box were used to construct the rat model of collagen-induced arthritis with wind-cold-dampness arthralgia syndrome. The rats were divided randomly into a control group, wind-cold-dampness syndrome model group, and high-, medium-, and low-dose ZFTG groups. The methotrexate (MTX) control group was treated with the corresponding drug intervention for 28 d. The joint temperature, pain threshold, joint swelling degree, and arthritis index (AI) score were measured. The production of C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), and rheumatoid factors (RFs) in the blood was detected by enzyme-linked immunosorbent assay. The protein expression of TLR2, TLR4, and NF-κB in synovial tissues was detected by Western blotting, and the mRNA expression of TLR2, TLR4, and NF-κB was detected by real-time polymerase chain reaction.

RESULTS

Compared with the model group, the joint temperature in each treatment group, the MTX control group, and MTX group recovered, the degree of foot swelling, pain threshold, AI score decreased, serum CRP, ESR, RF level and the levels of TLR2, TLR4, and NF-κB in synovial tissue were decreased (P < 0.05). Among them, the curative effect in the medium-dose and MTX groups was more evident (P < 0.01).

CONCLUSION

ZFTG has a significant effect on RA in rats, and its mechanism may involve regulating CRP levels, the ESR, and RFs via the TLR2/4-NF-κB signaling pathway.

摘要

目的

观察追风透骨胶囊（ZFTG）治疗佐剂型关节炎大鼠的疗效，探讨其作用机制，重点在于 Toll 样受体 2/4-核因子κB（TLR2/4-NF-κB）信号通路。

方法

采用Ⅱ型胶原和人工气候箱复合造模法，建立风寒湿痹证佐剂型关节炎大鼠模型，将大鼠随机分为空白组、风寒湿痹证模型组、ZFTG 高、中、低剂量组、甲氨蝶呤（MTX）对照组，各给药组给予相应药物干预 28 d，观察大鼠关节温度、痛阈值、关节肿胀度、关节炎指数（AI）评分的变化；酶联免疫吸附法检测大鼠血清 C 反应蛋白（CRP）、红细胞沉降率（ESR）、类风湿因子（RF）的含量；Western blot 法检测大鼠滑膜组织 TLR2、TLR4、NF-κB 蛋白表达，实时荧光定量聚合酶链反应（PCR）检测 TLR2、TLR4、NF-κB mRNA 表达。