Yang Yuantao, Hu Yiming, Shi Wen, Ma Huimin
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences Beijing 100190 China
University of Chinese Academy of Sciences Beijing 100049 China.
Chem Sci. 2020 Sep 25;11(47):12802-12806. doi: 10.1039/d0sc04537c.
Pantetheinase is an amidohydrolase that cleaves pantetheine into pantothenic acid and cysteamine. Functional studies have found that ubiquitous expression of this enzyme is associated with many inflammatory diseases. However, the lack of near-infrared fluorescence probes limits the better understanding of the functions of the enzyme. In this work, we have developed a new near-infrared fluorescence probe, CYLP, for bioimaging of pantetheinase by using pantothenic acid with a self-immolative linker as a recognition group. The probe produces a sensitive fluorescence off-on response at 710 nm to pantetheinase with a detection limit of 0.02 ng mL and can be used to image the intraperitoneal pantetheinase activity in mice . Moreover, with the probe we have observed that pantetheinase is significantly increased in the tissues of mouse inflammatory models as well as in the intestines of mice with inflammatory bowel disease. Therefore, CYLP may provide a convenient and intuitive tool for studying the role of pantetheinase in diseases.
泛硫乙胺酶是一种酰胺水解酶，可将泛硫乙胺裂解为泛酸和半胱胺。功能研究发现，这种酶的广泛表达与许多炎症性疾病有关。然而，缺乏近红外荧光探针限制了对该酶功能的深入了解。在这项工作中，我们开发了一种新的近红外荧光探针CYLP，通过使用带有自裂解连接子的泛酸作为识别基团，用于泛硫乙胺酶的生物成像。该探针对泛硫乙胺酶在710 nm处产生灵敏的荧光开-关响应，检测限为0.02 ng/mL，可用于成像小鼠腹腔内的泛硫乙胺酶活性。此外，使用该探针我们观察到，在小鼠炎症模型的组织以及炎症性肠病小鼠的肠道中，泛硫乙胺酶显著增加。因此，CYLP可能为研究泛硫乙胺酶在疾病中的作用提供一种方便直观的工具。
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