Wang Chenghong, Zhu Guicai, Yu Miaolin, Mi Xiufang, Qu Honghua
Department of Clinical Laboratory, Yantaishan Hospital, Yantai 264000, China.
Department of Surgery, Rizhao Hospital of TCM, Rizhao 276800, China.
J Oncol. 2021 May 27;2021:9923454. doi: 10.1155/2021/9923454. eCollection 2021.
Hepatocellular carcinoma (HCC) has been regarded as the fifth most common cancer worldwide with a low prognosis. miR-455 usually played the role of a tumor suppressor in multiple cancers. The aim of this study was to investigate the roles of miR-455 in HCC.
Cell viability and invasion were measured by CCK8 and Transwell assays. Luciferase reporter assay was performed to verify that miR-455 directly binds to the 3'-noncoding region (UTR) of RAB18 mRNA in Huh7 cells.
The expression of miR-455 was lower in HCC tissues and cell lines than in nontumor tissues and normal cell line, and downregulation of miR-455 was connected with worse outcome of HCC patients. miR-455 suppressed cell proliferation in vitro and in vivo, and it inhibited the abilities of cell invasion and EMT in HCC. RAB18 was upregulated in HCC tissues and cell lines, and the expression of RAB18 was regulated by miR-455. RAB18 reversed partial roles of miR-455 on cell viability and invasion in HCC.
miR-455 inhibited cell viability and invasion by directly targeting the 3'-UTR of RAB18 mRNA of hepatocellular carcinoma.
肝细胞癌（HCC）被认为是全球第五大常见癌症，预后较差。miR - 455在多种癌症中通常发挥肿瘤抑制作用。本研究旨在探讨miR - 455在HCC中的作用。
采用CCK8和Transwell实验检测细胞活力和侵袭能力。进行荧光素酶报告基因实验以验证miR - 455在Huh7细胞中直接与RAB18 mRNA的3'非编码区（UTR）结合。
HCC组织和细胞系中miR - 455的表达低于非肿瘤组织和正常细胞系，miR - 455的下调与HCC患者较差的预后相关。miR - 455在体外和体内均抑制细胞增殖，并抑制HCC细胞的侵袭和上皮 - 间质转化（EMT）能力。RAB18在HCC组织和细胞系中上调，且RAB18的表达受miR - 455调控。RAB18逆转了miR - 455对HCC细胞活力和侵袭的部分作用。
miR - 455通过直接靶向肝细胞癌RAB18 mRNA的3'UTR抑制细胞活力和侵袭。
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