Laboratorio de Genética Molecular y Bioquímica, Universidad Científica del Sur, Lima, Perú.
Instituto de Medicina Tropical «Alexander von Humboldt», Universidad Peruana Cayetano Heredia, Lima, Perú.
Rev Peru Med Exp Salud Publica. 2021 Jan-Mar;38(1):130-135. doi: 10.17843/rpmesp.2021.381.6727. Epub 2021 Jun 25.
The present report is the original description of bla TEM-176. The mechanisms of resistance to antimicrobial agents were determined in an enterotoxigenic Escherichia coli, determining the susceptibility to 22 antimicrobials classified in 15 different groups by agar diffusion and establishing the phylogenetic group, mechanisms of resistance and presence of Class 1 and 2 integrons. Integrons and β-lactam resistance genes were sequenced. The isolate, belonging to phylogenetic group A, showed the presence of resistance or diminished susceptibility to a ampicillin, amoxicillin plus clavulanic acid, nalidíxic acid, ciprofloxacin, streptomycin, kanamycin, tetracycline, trimethoprim, sulfisoxazole, cotrimoxazole, azithromycin and nitrofurantoin, carrying bla TEM, aadA1/2, aphA1, sul3, tet(A) and a Class 2 integron containing a dfrA1 gene. Quinolone resistance was related to the substitution Ser83Ala. The TEM sequencing showed the presence of the new substitution Ala222Val, which led to the description of the new β-lactamase bla TEM-176.
本报告是 blaTEM-176 的原始描述。通过琼脂扩散法确定了 22 种抗菌药物的敏感性，将其分为 15 个不同组别，从而确定了肠产毒型大肠埃希菌对抗菌药物的耐药机制，并确定了其进化群、耐药机制以及 1 类和 2 类整合子的存在。整合子和β-内酰胺类耐药基因进行了测序。该分离株属于 A 进化群，对氨苄西林、阿莫西林加克拉维酸、萘啶酸、环丙沙星、链霉素、卡那霉素、四环素、甲氧苄啶、磺胺甲噁唑、复方磺胺甲噁唑、阿奇霉素和呋喃妥因的耐药性或敏感性降低，携带 blaTEM、aadA1/2、aphA1、sul3、tet(A)和一个包含 dfrA1 基因的 2 类整合子。喹诺酮类耐药与 Ser83Ala 的取代有关。TEM 测序显示存在新的取代 Ala222Val，这导致了新的β-内酰胺酶 blaTEM-176 的描述。
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