Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Ciszewskiego Str. 8, 02-786, Warsaw, Poland.
Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Ciszewskiego Str. 8, 02-786, Warsaw, Poland.
Vet Microbiol. 2021 Aug;259:109160. doi: 10.1016/j.vetmic.2021.109160. Epub 2021 Jun 18.
High-level mupirocin resistance (HLMR) is determined by the plasmid-located ileS2 gene flanked by two copies of the insertion sequence 257 (IS257). The molecular epidemiology of high-level mupirocin-resistant isolates could be assessed by the determination of their IS257-ileS2 spacer regions conformation. In this study, 188 isolates of methicillin-resistant staphylococci were subjected to the detection of HLMR, and analysis of the conformation of the IS257-ileS2 spacer regions. Mupirocin resistance was detected in five (2,6%) isolates, among which two were recognized as Staphylococcus pseudintermedius, two as Staphylococcus haemolyticus, and one as Staphylococcus aureus. High-level mupirocin resistance was revealed by the agar disk diffusion method, and MIC values, and was confirmed by the detection of the ileS2 gene. The conformations of the IS257-ileS2 spacer regions were homologous in two S. haemolyticus strains tested. The remaining three isolates showed diverse IS257-ileS2 conformations. The results of this study indicate that HLMR occasionally occurs in staphylococci isolated from companion animals. The heterogeneity and the homogeneity of the IS257-ileS2 spacer regions confirm that the ileS2 gene spread among staphylococci of animal origin by the transfer of different as well as the same plasmids. Surveillance of the occurrence of mupirocin resistance and molecular characterization of resistant isolates are strongly recommended due to the possibility of plasmid-located resistance gene transfer between staphylococci.
高水平米诺环素耐药（HLMR）由质粒定位的 ileS2 基因决定，该基因两侧是两个插入序列 257（IS257）。通过测定 IS257-ileS2 间隔区的构象，可以评估高水平米诺环素耐药分离株的分子流行病学。在这项研究中，对 188 株耐甲氧西林葡萄球菌进行了 HLMR 检测和 IS257-ileS2 间隔区构象分析。在五个（2.6%）分离株中检测到米诺环素耐药，其中两个被鉴定为中间葡萄球菌，两个为溶血葡萄球菌，一个为金黄色葡萄球菌。琼脂扩散法、MIC 值和 ileS2 基因检测均显示高水平米诺环素耐药。IS257-ileS2 间隔区的构象在两个测试的溶血葡萄球菌菌株中是同源的。其余三个分离株表现出不同的 IS257-ileS2 构象。本研究结果表明，HLMR 偶尔发生在来自伴侣动物的葡萄球菌中。IS257-ileS2 间隔区的异质性和同质性证实，ileS2 基因通过不同和相同质粒的转移在动物来源的葡萄球菌中传播。由于质粒定位的耐药基因在葡萄球菌之间转移的可能性，强烈建议对米诺环素耐药的发生进行监测，并对耐药分离株进行分子特征分析。
