Brinza Ion, Ayoub Iriny M, Eldahshan Omayma A, Hritcu Lucian
Department of Biology, Faculty of Biology, Alexandru Ioan Cuza University of Iasi, 700506 Iasi, Romania.
Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Abbassia, Cairo 11566, Egypt.
Plants (Basel). 2021 Jun 18;10(6):1245. doi: 10.3390/plants10061245.
Baicalein 5,6-dimethyl ether, a bioactive flavonoid isolated for the first time from , was explored for its capability to relieve memory deficits and decrease oxidative stress. We examined the neuropharmacological effects of baicalein 5,6-dimethyl ether on scopolamine (Sco)-induced zebrafish () anxiety, amnesia, and brain oxidative stress and attempted to elucidate the underlying mechanisms. Anxiety-like behavior, exploratory behavior, and memory performance were measured using novel tank-diving test (NTT), Y-maze, and novel object recognition (NOR) tests. For 10 days, baicalein 5,6-dimethyl ether (1, 3, and 5 µg/L) was administered through immersion, whereas Sco (100 μM) was delivered 30 min before behavioral tests. Treatment with baicalein 5,6-dimethyl ether reduced anxiety and memory impairment, and increased exploratory behavior in specific tests, along with significant protection from neuronal oxidative stress in the brain tissue of Sco-treated zebrafish. Antioxidant and anti-acetylcholinesterase (AChE) activities of baicalein 5,6-dimethyl ether in the Sco-induced zebrafish were further confirmed using in vivo assays. In Sco-treated zebrafish, baicalein 5,6-dimethyl ether regulated cholinergic function by inhibiting AChE activity. Baicalein 5,6-dimethyl ether may be a promising candidate compound for treating anxiety and amnesia by restoring cholinergic activity and reducing brain oxidative stress, according to our findings.
首次从 中分离出的生物活性黄酮类化合物黄芩素5,6-二甲醚，对其缓解记忆缺陷和降低氧化应激的能力进行了研究。我们研究了黄芩素5,6-二甲醚对东莨菪碱（Sco）诱导的斑马鱼焦虑、失忆和脑氧化应激的神经药理学作用，并试图阐明其潜在机制。使用新水箱潜水试验（NTT）、Y迷宫试验和新物体识别（NOR）试验来测量类似焦虑行为、探索行为和记忆表现。连续10天，通过浸泡给予黄芩素5,6-二甲醚（1、3和5μg/L），而在行为测试前30分钟给予Sco（100μM）。黄芩素5,6-二甲醚治疗可减轻焦虑和记忆障碍，并在特定试验中增加探索行为，同时对Sco处理的斑马鱼脑组织中的神经元氧化应激有显著保护作用。使用体内试验进一步证实了黄芩素5,6-二甲醚在Sco诱导的斑马鱼中的抗氧化和抗乙酰胆碱酯酶（AChE）活性。在Sco处理的斑马鱼中，黄芩素5,6-二甲醚通过抑制AChE活性来调节胆碱能功能。根据我们的研究结果，黄芩素5,6-二甲醚可能是一种有前途的候选化合物，可通过恢复胆碱能活性和降低脑氧化应激来治疗焦虑和失忆。
