一种基于活性的荧光传感器，用于检测活细胞中的苯酚磺基转移酶SULT1A1。
An activity-based fluorescent sensor for the detection of the phenol sulfotransferase SULT1A1 in living cells.
作者信息
Baglia Regina A, Mills Kira R, Mitra Koushambi, Tutol Jasmine N, Ball Darby, Page Kierstin M, Kallu Jyothi, Gottipolu Sriharika, D'Arcy Sheena, Nielsen Steven O, Dodani Sheel C
机构信息
Department of Chemistry and Biochemistry, The University of Texas at Dallas Richardson TX 75080 USA
出版信息
RSC Chem Biol. 2021 Mar 15;2(3):830-834. doi: 10.1039/d0cb00231c.
Human phenol sulfotransferases mediate the transfer of a sulfuryl moiety from the activated sulfate donor PAPS to hydroxy-containing substrates, altering substrate solubility and charge to affect phase II metabolism and cell signaling. Here, we present the development, computational modeling, enzymology, and biological application of STS-3, an activity-based fluorescent sensor for the SULT1A1 isoform.
人类酚磺基转移酶介导将硫酸酰基部分从活化的硫酸供体PAPS转移至含羟基的底物，改变底物的溶解度和电荷，从而影响II相代谢和细胞信号传导。在此，我们展示了STS-3的开发、计算建模、酶学及生物学应用，STS-3是一种针对SULT1A1同工型的基于活性的荧光传感器。
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