Beguin Y, Huebers H A, Josephson B, Finch C A
Department of Medicine, University of Washington, Seattle 98195.
Proc Natl Acad Sci U S A. 1988 Jan;85(2):637-40. doi: 10.1073/pnas.85.2.637.
Antigenic material in rat plasma reacting with rat transferrin receptor antibodies was identified as an intact receptor molecule complexed with transferrin. Plasma transferrin receptors were measured by ELISA in rats of different age and sex, of different iron status, with different degrees of erythropoiesis, and with inflammation. An inverse relationship between iron status and receptor number was found, whereas a direct relationship existed between erythropoiesis and receptors. These changes in receptor number can be explained by assuming that the number of tissue receptors determined the number of plasma receptors and that the erythroid cells possessed most of the body's receptors. Increases in plasma receptors lagged behind the appearance of circulating reticulocytes, suggesting that receptors were released to the plasma during the terminal phase of erythrocyte maturation.
大鼠血浆中与大鼠转铁蛋白受体抗体发生反应的抗原物质被鉴定为与转铁蛋白复合的完整受体分子。采用酶联免疫吸附测定法(ELISA)检测不同年龄、性别、铁状态、红细胞生成程度以及有炎症的大鼠血浆中转铁蛋白受体。结果发现铁状态与受体数量呈负相关,而红细胞生成与受体呈正相关。受体数量的这些变化可以通过以下假设来解释,即组织受体的数量决定了血浆受体的数量,且红细胞系细胞拥有机体大部分的受体。血浆受体的增加滞后于循环中网织红细胞的出现,这表明受体是在红细胞成熟的终末期释放到血浆中的。