在日本人中，糖蛋白变体和针对 Hil 和 MINY 抗原的同种抗体的频率。

Frequencies of glycophorin variants and alloantibodies against Hil and MINY antigens in Japanese.

机构信息

Japanese Red Cross Kanto-Koshinetsu Block Blood Center, Tokyo, Japan.

Japanese Red Cross Central Blood Institute, Tokyo, Japan.

出版信息

Vox Sang. 2022 Jan;117(1):94-98. doi: 10.1111/vox.13121. Epub 2021 Jul 6.

DOI:10.1111/vox.13121

PMID:34227688

Abstract

BACKGROUND AND OBJECTIVES

Antigens of the MNS blood group system are expressed on the red blood cell (RBC) membrane on glycophorin A (GPA) and glycophorin B (GPB) or on hybrid molecules of GPA and GPB. This study investigated the distribution of glycophorin variants and alloantibodies against Hil and MINY among Japanese individuals.

METHODS

Mi(a+) or Hil+ RBCs were screened using an automated blood grouping machine (PK7300) with monoclonal anti-Mi or polyclonal anti-Hil. Glycophorin variants were defined by serology with monoclonal antibodies against Mi , Vw, MUT and Mur, and polyclonal antibodies against Hil, MINY and Hop + Nob (KIPP). The glycophorin variants were further confirmed by immunoblotting and Sanger sequencing. Alloanti-Hil and alloanti-MINY in the plasma were screened using GP.Hil RBCs in an antiglobulin test. The specificity of anti-Hil or anti-MINY was assessed using GP.Hil (Hil+MINY+) and GP.JL (Hil-MINY+) RBCs.

RESULTS

The GP.HF, GP.Mur, GP.Hut, GP.Vw, GP.Kip and GP.Bun frequencies in 1 005 594 individuals were 0·0357%, 0·0256%, 0·0181%, 0·0017%, 0·0009% and 0·0007%, respectively. GP.Hil was found in as four of the 13 546 individuals (0·0295%). Of 137 370 donors, 10 had anti-Hil (0·0073%) and three had anti-MINY (0·0022%).

CONCLUSIONS

Glycophorin variants were relatively rare in Japanese individuals, with the major variants being GP.HF (0·0357%), GP.Hil (0·0295%) and GP.Mur (0·0256%). Only one example of anti-MINY was previously reported, but we found three more in this study.

摘要

背景与目的

MNS 血型系统的抗原在红细胞（RBC）膜上的糖蛋白 A（GPA）和糖蛋白 B（GPB）上表达，或在 GPA 和 GPB 的杂交分子上表达。本研究调查了日本人中糖蛋白变体和针对 Hil 和 MINY 的同种异体抗体的分布。

方法

使用带有抗 Mi 单克隆抗体或多克隆抗 Hil 的自动血型鉴定机（PK7300）筛选 Mi(a+)或 Hil+RBC。糖蛋白变体通过与针对 Mi、Vw、MUT 和 Mur 的单克隆抗体以及针对 Hil、MINY 和 Hop+Nob（KIPP）的多克隆抗体进行血清学定义。糖蛋白变体通过免疫印迹和 Sanger 测序进一步确认。使用抗球蛋白试验中的 GP.Hil RBC 筛选血浆中的同种异体抗 Hil 和同种异体抗 MINY。使用 GP.Hil（Hil+MINY+）和 GP.JL（Hil-MINY+）RBC 评估抗 Hil 或抗 MINY 的特异性。

结果

在 1 005 594 个人中，GP.HF、GP.Mur、GP.Hut、GP.Vw、GP.Kip 和 GP.Bun 的频率分别为 0.0357%、0.0256%、0.0181%、0.0017%、0.0009%和 0.0007%。在 13 546 个人中发现了四个 GP.Hil（0.0295%）。在 137 370 名供体中，有 10 名抗 Hil（0.0073%），3 名抗 MINY（0.0022%）。