利用叠氮-炔基环加成反应，通过 NaSO 介导的可裂解亲和标签合成，对 O-GlcNAc 修饰的蛋白质进行标记。
Synthesis of NaSO mediated cleavable affinity tag for labeling of O-GlcNAc modified proteins via azide-alkyne cycloaddition.
机构信息
Joint National Laboratory for Antibody Drug Engineering, The First Affiliated Hospital of Henan University, School of Basic Medicine Science, Henan University, 475004 Kaifeng, China; State Key Laboratory of Medicinal Chemical Biology, Nankai University, Haihe Education Park, 38 Tongyan Road, Tianjin 300353, China.
Joint National Laboratory for Antibody Drug Engineering, The First Affiliated Hospital of Henan University, School of Basic Medicine Science, Henan University, 475004 Kaifeng, China.
出版信息
Bioorg Med Chem Lett. 2021 Sep 15;48:128244. doi: 10.1016/j.bmcl.2021.128244. Epub 2021 Jul 3.
A facile and convergent procedure for the synthesis of azobenzene-based probe was reported, which could selectively release interested proteins conducted with sodium dithionite. Besides, the cleavage efficiency is closely associated with the structural features, in which an ortho-hydroxyl substituent is necessary for reactivity. In addition, the azobenzene tag applied in the AcGlcNAz-labled proteins demonstrated high efficiency and selectivity in comparison with Biotin-PEG-Alkyne, which provides a useful platform for enrichment of any desired bioorthogonal proteomics.
本文报道了一种简便、有效的基于偶氮苯的探针合成方法，该探针能够选择性地释放二硫苏糖醇引发的目标蛋白。此外，这种切割效率与结构特征密切相关，其中邻位羟基取代基是反应性所必需的。此外，与 Biotin-PEG-Alkyne 相比，偶氮苯标签在 AcGlcNAz 标记蛋白中的应用具有更高的效率和选择性，为任何所需的生物正交蛋白质组学的富集提供了一个有用的平台。
Zotero 插件