基于咪唑并[1,2-a]吲哚荧光团的新型 pH 响应荧光探针的制备及其在酿酒酵母中极低 pH 检测中的应用。
Preparation of a Novel pH-responsive Fluorescent Probe Based on an Imidazo[1,2-a]indole Fluorophore and its Application in Detecting Extremely Low pH in Saccharomyces cerevisiae.
机构信息
School of Pharmacy, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, Shandong, 271016, People's Republic of China.
Shanghai Fengxian Central Hospital, Shanghai, 201400, People's Republic of China.
出版信息
J Fluoresc. 2021 Sep;31(5):1219-1225. doi: 10.1007/s10895-021-02739-8. Epub 2021 Jul 13.
A novel pH-responsive probe based on an imidazo[1,2-a]indole fluorophore architecture is reported. The probe was highly selective to strongly acidic pH (pK = 3.56) with high sensitivity and a fast response time (within 30 s). The probe did not demonstrate any fluorescence changes in the presence of interfering metal ions, and it featured excellent reversibility under strongly acidic conditions. The mechanism of detection of the probe was determined to be based on intramolecular charge transfer (ICT) at different pH. The probe was also able to be used for imaging for detecting acidic pH in Saccharomyces cerevisiae.
报告了一种基于咪唑并[1,2-a]吲哚荧光团结构的新型 pH 响应探针。该探针对强酸性 pH（pK = 3.56）具有高选择性，灵敏度高，响应时间快（在 30 秒内）。在存在干扰金属离子的情况下，探针没有显示出任何荧光变化，并且在强酸性条件下具有出色的可逆性。检测探针的机制被确定为基于不同 pH 值下的分子内电荷转移（ICT）。该探针还可用于成像，以检测酿酒酵母中的酸性 pH 值。
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