Lippke J A, Strzempko M N, Raia F F, Simon S L, French C K
BioTechnica Diagnostics, Cambridge, Massachusetts 02138.
Appl Environ Microbiol. 1987 Oct;53(10):2588-9. doi: 10.1128/aem.53.10.2588-2589.1987.
A method for obtaining high-molecular-weight chromosomal DNA from Bacteroides intermedius and Bacteroides gingivalis is described. This technique is a modification of the guanidine isothiocyanate isolation procedure for RNA and should be useful for isolating intact DNA from organisms with high endogenous nuclease activity.
描述了一种从中间拟杆菌和牙龈拟杆菌中获取高分子量染色体DNA的方法。该技术是对用于RNA的异硫氰酸胍分离程序的改进，应该有助于从具有高内源性核酸酶活性的生物体中分离完整的DNA。
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