一种用于多次输血患者KEL1和KEL2基因分型的简易多重聚合酶链反应-序列特异性引物分析方法
An Easy Multiplex PCR-SSP Assay for the Genotyping of KEL1 and KEL2 in Multi-transfused Patients.
作者信息
Zarghamian Parinaz, Pourshadlou Maryam, Mousavi Hosseini Kamran, Sarem Fariba, Shahabi Majid
机构信息
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Hemmat Expressway, IBTO Building, Tehran, 1449613111 Iran.
出版信息
Indian J Hematol Blood Transfus. 2021 Jul;37(3):489-491. doi: 10.1007/s12288-020-01389-3. Epub 2021 Jan 1.
Kell blood group system consists of 34 antigens. KEL1 and KEL2 are the most clinically important antigens of this system, causing hemolytic disease of the fetus and newborn (HDFN) and transfusion reaction. A total of 200 samples from blood donors were tested serologically for the presence of KEL1 and KEL2 antigens on erythrocytes. Genomic DNA was analyzed by PCR-SSP method to determine the Kell genotype. A multiplex PCR-SSP assay was designed and tested to genotype KEL1/KEL2 alleles in a single reaction. PCR genotyping revealed samples as; KEL2/KEL2 (93.5%) and KEL1/KEL2 (6.5%), while no sample determined as KEL1/KEL1. A 100% concordance observed between PCR and serological results. Multiplex PCR accurately diagnosed Kell genotype. Kell blood group genotyping by PCR-SSP can be used as an alternative method, especially in multi-transfused patients where serological findings are ambiguous.
凯尔血型系统由34种抗原组成。KEL1和KEL2是该系统中临床上最重要的抗原，可导致胎儿及新生儿溶血病（HDFN）和输血反应。对200份献血者样本进行红细胞上KEL1和KEL2抗原存在情况的血清学检测。采用PCR-SSP方法分析基因组DNA以确定凯尔基因型。设计并测试了一种多重PCR-SSP检测方法，以便在单一反应中对KEL1/KEL2等位基因进行基因分型。PCR基因分型结果显示样本为：KEL2/KEL2（93.5%）和KEL1/KEL2（6.5%），未检测到KEL1/KEL1样本。PCR结果与血清学结果完全一致。多重PCR准确诊断了凯尔基因型。通过PCR-SSP进行凯尔血型基因分型可作为一种替代方法，尤其适用于血清学结果不明确的多次输血患者。
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