通过协作试验对 SPE 和 LC-MS/MS-A 方法进行验证，测定番茄、小麦和葵花籽中的交链孢毒素。
Determination of Alternaria Toxins in Tomato, Wheat, and Sunflower Seeds by SPE and LC-MS/MS-A Method Validation Through a Collaborative Trial.
机构信息
European Commission, Joint Research Centre (JRC), Geel, Belgium.
Mertcontroll Ltd., Szabadság street 13, 2144 Kerepes, Hungary.
出版信息
J AOAC Int. 2022 Feb 4;105(1):80-94. doi: 10.1093/jaoacint/qsab094.
BACKGROUND
Alternaria toxins are ubiquitous contaminants in highly consumed food products. Therefore, they are candidates to be regulated by EU legislation. In this context, the availability of reliable analytical methods is a keystone both for protecting the health of citizens and smooth functioning of the European market.
OBJECTIVE
This paper describes an advanced LC-MS/MS method based on isotope dilution quantification suitable for the determination of altenuene, alternariol, alternariol monomethyl ether, tenuazonic acid, and tentoxin in tomato puree, wheat, and sunflower seeds.
METHODS
The method has been validated in an interlaboratory study that included the analysis of both spiked and naturally contaminated food commodities. Twenty-three participants contributed with analytical data.
RESULTS
The average recoveries and relative standard deviations for repeatability and reproducibility obtained across the tested matrixes were: 97, 8.0, and 23%, for altenuene, respectively; 95, 9.2, and 17% for alternariol, respectively; 98, 6.4, and 13% for alternariol monomethyl ether, respectively; 97, 4.2, and 9.3% for tenuazonic acid, respectively; and 102, 5.6, and 15% for tentoxin, respectively. The method enabled the determination of all tested Alternaria toxins close to or below 1 µg/kg.
CONCLUSION
Overall, the method showed a satisfactory trueness and precision, complying with the requirements for the monitoring of mycotoxins in food in the EU. It is currently under evaluation by the European Committee for Standardization for adoption as a standard method.
HIGHLIGHTS
Isotope dilution mass spectrometry method for the determination of Alternaria toxins in food.
背景
链格孢菌毒素是高度消费的食品中普遍存在的污染物。因此，它们是欧盟法规监管的候选物。在这种情况下，可靠的分析方法的可用性是保护公民健康和欧洲市场顺利运作的关键。
目的
本文描述了一种基于同位素稀释定量的先进 LC-MS/MS 方法，适用于番茄酱、小麦和葵花籽中 alternuene、altenariol、altenariol monomethyl ether、tenuazonic acid 和 tentoxin 的测定。
方法
该方法在一项包括对加标和天然污染食品进行分析的实验室间研究中得到了验证。共有 23 名参与者提供了分析数据。
结果
在所测试的基质中，平均回收率和重复性及再现性的相对标准偏差分别为：altenuene 为 97%、8.0%和 23%；alternariol 分别为 95%、9.2%和 17%；altenariol monomethyl ether 分别为 98%、6.4%和 13%；tenuazonic acid 分别为 97%、4.2%和 9.3%；tentoxin 分别为 102%、5.6%和 15%。该方法能够在接近或低于 1μg/kg 的水平下测定所有测试的链格孢菌毒素。
结论
总的来说，该方法具有令人满意的准确性和精密度，符合欧盟食品中霉菌毒素监测的要求。它目前正在由欧洲标准化委员会进行评估，以作为标准方法通过。
亮点
用于测定食品中链格孢菌毒素的同位素稀释质谱法。
Zotero 插件