Novosibirsk Institute of Organic Chemistry, 9 Lavrentiev Avenue, 630090 Novosibirsk, Russia.
Department of Physics, Novosibirsk State University, 630090 Novosibirsk, Russia.
Molecules. 2021 Jul 17;26(14):4340. doi: 10.3390/molecules26144340.
Nitric oxide (NO) is an important signaling molecule involved in a wide range of physiological and pathological processes. Fluorescent imaging is a useful tool for monitoring NO concentration, which could be essential in various biological and biochemical studies. Here, we report the design of a novel small-molecule fluorescent probe based on 9(10)acridone moiety for nitric oxide sensing. 7,8-Diamino-4-carboxy-10-methyl-9(10)acridone reacts with NO in aqueous media in the presence of O, yielding a corresponding triazole derivative with fivefold increased fluorescence intensity. The probe was shown to be capable of nitric oxide sensing in living Jurkat cells.
一氧化氮（NO）是一种重要的信号分子，参与广泛的生理和病理过程。荧光成像技术是监测 NO 浓度的一种有用工具，这在各种生物和生化研究中至关重要。在这里，我们报告了一种基于 9(10)吖啶酮部分的新型小分子荧光探针的设计，用于检测一氧化氮。7,8-二氨基-4-羧基-10-甲基-9(10)吖啶酮在含有 O 的水介质中与 NO 反应，生成相应的三唑衍生物，荧光强度增加五倍。该探针被证明能够在活 Jurkat 细胞中检测到一氧化氮。
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