人精浆前列腺小体中的磷脂酶A2活性

Phospholipase A2 activity in prostasomes from human seminal plasma.

作者信息

Lindahl M, Tagesson C, Ronquist G

机构信息

Department of Occupational Medicine, Linköping University Hospital, Sweden.

出版信息

Urol Int. 1987;42(5):385-9. doi: 10.1159/000281999.

DOI:10.1159/000281999

PMID:3433587

Abstract

Phospholipase A2 (PLA2) activity was measured and partially characterized in prostasomes isolated from human seminal plasma. The results show high PLA2 activity in seminal plasma with a threefold enrichment in the isolated prostasomes. Highest activity was found at pH 8.0 and 10.5, and there was an absolute requirement for calcium with almost total inhibition of PLA2 activity in the presence of 1 mM EDTA. Analysis with two-dimensional gel electrophoresis of prostasome material revealed a complex protein pattern with most of the proteins in the molecular weight interval of 10,000-90,000 daltons. The possible role of PLA2 in prostasomes is discussed.

摘要

对从人类精浆中分离出的前列腺小体中的磷脂酶A2（PLA2）活性进行了测定，并对其部分特性进行了表征。结果显示，精浆中PLA2活性较高，分离出的前列腺小体中该活性富集了三倍。在pH 8.0和10.5时活性最高，并且对钙有绝对需求，在存在1 mM乙二胺四乙酸（EDTA）的情况下，PLA2活性几乎完全受到抑制。对前列腺小体物质进行二维凝胶电泳分析，揭示了一种复杂的蛋白质图谱，大多数蛋白质的分子量在10,000 - 90,000道尔顿之间。文中讨论了PLA2在前列腺小体中的可能作用。