School of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, P. R. China.
Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, P. R. China.
Food Funct. 2021 Sep 20;12(18):8615-8625. doi: 10.1039/d1fo01405f.
Three peptides containing three glutamic acid (Glu) residues at different positions derived from Antarctic krill were obtained to investigate their iron-binding properties, digestive stability, and effectiveness on enhancing iron solubility and absorption. Results indicated that Fe bound to the carbonyl, carboxyl, or hydroxyl groups of DELEDSLER, EEEFDATR, and DTDSEEEIR at stoichiometric ratios of 0.453, 0.466, and 0.490, respectively. DTDSEEEIR with three consecutive Glu in the middle of the sequence possessed higher iron-binding ability and iron release potential than EEEFDATR with three consecutive Glu in the N-terminal, and DELEDSLER with three discontinuous Glu showed the lowest values. Although EEEFDATR showed remarkably lower digestion stability than DTDSEEEIR, the effect of EEEFDATR-iron on iron solubility and absorption was comparable to that of DTDSEEEIR-iron, but better than that of DELEDSLER-iron and FeSO. Thus, peptides with consecutive Glu have the potential as an effective iron carrier to improve iron absorption.
获得了三种源自南极磷虾的含有三个不同位置谷氨酸（Glu）残基的肽，以研究它们的铁结合特性、消化稳定性以及提高铁溶解度和吸收的效果。结果表明，Fe 与 DELEDSLER、EEEFDATR 和 DTDSEEEIR 的羰基、羧基或羟基以化学计量比 0.453、0.466 和 0.490 结合。在序列中间有三个连续 Glu 的 DTDSEEEIR 具有比 N 末端有三个连续 Glu 的 EEEFDATR 更高的铁结合能力和铁释放潜力，而具有三个不连续 Glu 的 DELEDSLER 则显示出最低的值。尽管 EEEFDATR 的消化稳定性明显低于 DTDSEEEIR，但 EEEFDATR-铁对铁溶解度和吸收的影响与 DTDSEEEIR-铁相当，但优于 DELEDSLER-铁和 FeSO。因此，具有连续 Glu 的肽有潜力作为有效的铁载体来提高铁的吸收。
