首例格氏假单胞菌噬菌体PMBT3的特性分析

Sprotte Sabrina, Brinks Erik, Wagner Natalia, Kropinski Andrew M, Neve Horst, Franz Charles M A P

Department of Microbiology and Biotechnology, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Hermann-Weigmann-Str. 1, 24103, Kiel, Germany.

Departments of Food Science and Pathobiology, University of Guelph, 50 Stone Road E, Guelph, Ontario, N1G 2W1, Canada.

Arch Virol. 2021 Oct;166(10):2887-2894. doi: 10.1007/s00705-021-05173-0. Epub 2021 Aug 4.

The complete genome sequence of the virulent bacteriophage PMBT3, isolated on the proteolytic Pseudomonas grimontii strain MBTL2-21, showed no significant similarity to other known phage genome sequences, making this phage the first reported to infect a strain of P. grimontii. Electron microscopy revealed PMBT3 to be a member of the family Siphoviridae, with notably long and flexible whiskers. The linear, double-stranded genome of 87,196 bp has a mol% G+C content of 60.4 and contains 116 predicted protein-encoding genes. A putative tellurite resistance (terB) gene, originally reported to occur in the genome of a bacterium, was detected in the genome of phage PMBT3.

在蛋白水解的格氏假单胞菌菌株MBTL2 - 21上分离出的烈性噬菌体PMBT3的全基因组序列，与其他已知噬菌体基因组序列没有显著相似性，这使得该噬菌体成为首个报道的能感染格氏假单胞菌菌株的噬菌体。电子显微镜显示PMBT3属于长尾噬菌体科，具有特别长且灵活的须。其87,196 bp的线性双链基因组的G + C含量为60.4 mol%，包含116个预测的蛋白质编码基因。在噬菌体PMBT3的基因组中检测到一个假定的亚碲酸盐抗性（terB）基因，该基因最初报道存在于一种细菌的基因组中。