氟通过调节 AMPK/p38 信号通路对中性粒细胞胞外诱捕网形成的影响。
Effects of Fluorine on Neutrophil Extracellular Trap Formation through Regulating AMPK/p38 Signaling Pathway.
机构信息
Department of Nephrology, The Second Hospital of Jilin University, Changchun 130021, China.
College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.
出版信息
Oxid Med Cell Longev. 2021 Aug 2;2021:6693921. doi: 10.1155/2021/6693921. eCollection 2021.
Fluorine is an important trace element that is widely dispersed, and studies showed that fluorine could cause severe toxicity to fish. The aim of this study was to investigate the effects of fluorine on neutrophil extracellular trap (NET) formation in common carp and clarify the possible mechanism. The neutrophils were isolated and exposed to 0.25, 0.5, or 1 mM sodium fluoride (NaF). The results showed that NaF could induce the formation of NETs which exhibited a DNA-based network structure modified with histones and myeloperoxidase (MPO). Furthermore, NaF led to the production of reactive oxygen species (ROS) in neutrophils. Western blot results showed that NaF significantly increased the phosphorylation of AMPK and p38. In addition, our results showed that NaF-induced NET formation could be inhibited by an AMPK or p38 inhibitor. In conclusion, our results showed that NaF induced NET formation in neutrophils through regulation of the AMPK/p38 signaling pathway.
氟是一种广泛分布的重要微量元素，研究表明氟对鱼类有严重毒性。本研究旨在探讨氟对鲤鱼中性粒细胞胞外陷阱（NET）形成的影响，并阐明可能的机制。分离中性粒细胞并暴露于 0.25、0.5 或 1mM 氟化钠（NaF）。结果表明，NaF 可诱导形成 NET，其具有用组蛋白和髓过氧化物酶（MPO）修饰的基于 DNA 的网络结构。此外，NaF 导致中性粒细胞中活性氧（ROS）的产生。Western blot 结果表明，NaF 显著增加了 AMPK 和 p38 的磷酸化。此外，我们的结果表明，NaF 诱导的 NET 形成可以被 AMPK 或 p38 抑制剂抑制。总之，我们的结果表明，NaF 通过调节 AMPK/p38 信号通路诱导中性粒细胞中 NET 的形成。
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