生物合成人生长激素二聚体形式的化学、物理和生物学特性

Chemical, physical, and biological characterization of a dimeric form of biosynthetic human growth hormone.

作者信息

Becker G W, Bowsher R R, Mackellar W C, Poor M L, Tackitt P M, Riggin R M

机构信息

Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285.

出版信息

Biotechnol Appl Biochem. 1987 Dec;9(6):478-87. doi: 10.1111/j.1470-8744.1987.tb00491.x.

DOI:10.1111/j.1470-8744.1987.tb00491.x

PMID:3440058

Abstract

A dimer of biosynthetic human growth hormone (HGH) has been isolated and characterized. This entity, which is the predominant dimeric species in biosynthetic HGH, is chemically identical to monomeric HGH and exists in a noncovalent dimeric form which is dissociated to monomeric HGH on polyacrylamide electrophoresis gels or in aqueous solutions containing 30% acetonitrile. This substance, found in all production lots of pituitary HGH, biosynthetic HGH, and biosynthetic methionyl HGH examined, is much less biopotent than monomeric HGH and can be distinguished from monomeric HGH by a monoclonal antibody. These data demonstrate that polyacrylamide gel electrophoresis is not a valid method for measuring this dimer and that size-exclusion chromatography under aqueous conditions is required.

摘要

已分离并鉴定出生物合成人生长激素（HGH）的二聚体。该实体是生物合成HGH中的主要二聚体物种，在化学上与单体HGH相同，以非共价二聚体形式存在，在聚丙烯酰胺电泳凝胶上或含有30%乙腈的水溶液中会解离为单体HGH。在所有检测的垂体HGH、生物合成HGH和生物合成甲硫氨酰HGH生产批次中均发现了这种物质，其生物活性远低于单体HGH，并且可以通过单克隆抗体与单体HGH区分开来。这些数据表明，聚丙烯酰胺凝胶电泳不是测量这种二聚体的有效方法，需要在水性条件下进行尺寸排阻色谱分析。