Skowerski Tomasz, Nabrdalik Katarzyna, Kwiendacz Hanna, Pajak Maciej, Ochała Andrzej, Mizia-Stec Katarzyna, Gąsior Zbigniew, Gumprecht Janusz
Department of Cardiology, School of Health Sciences, Medical University of Silesia, Katowice, Poland.
Department of Internal Medicine, Diabetology and Nephrology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, Poland.
Postepy Kardiol Interwencyjnej. 2021 Jun;17(2):187-192. doi: 10.5114/aic.2021.107498. Epub 2021 Jul 9.
It is well known that chronic hyperglycemia or chronic inflammation leads to both FOXO1 and Ang-2 gene (ANGPT2) expression induction in endothelial cells. ANGPT2 and FOXO1 relative gene expression in peripheral blood cells in diabetes and myocardial ischemia were not researched extensively.
Our objective was to evaluate ANGPT2 and FOXO1 gene expression in peripheral blood cells in patients with non-ST elevation myocardial infarction (NSTEMI), both with and without type 2 diabetes mellitus (T2DM), and compare them to the results obtained from T2DM and control subjects.
This was a multi-center, prospective study of 138 NSTEMI patients with/without T2DM, T2DM and a control group. FOXO1, ANGPT2, TBP (TATA box binding protein - as a reference gene) gene expression levels in peripheral blood cells were measured in each patient. Electrocardiography and echocardiography with assessment of ejection fraction (EF) were performed. Patients with NSTEMI underwent urgent (< 24 h) coronarography and the SYNTAX score and GRACE 2.0 score were calculated.
The ANGPT2 gene relative expression in buffy coat in the analyzed samples was very low and detectable only in 11 patients from all groups (8.66% of all patients). The level of FOXO1 gene relative expression was significantly higher in patients with NSTEMI (median relative expression = 1.39) than in non-NSTEMI patients (median = 1.09) ( = 1578, < 0.05) regardless of the presence of T2DM. The FOXO1 gene relative expression was not correlated with GRACE 2.0 score or SYNTAX score of NSTEMI patients. We did not observe any significant change in FOXO1 gene expression after successful angioplasty.
On the basis of our results we can conclude that analyzing the ANGPT2 gene relative expression in peripheral blood cells has no role in assessment of CAD complexity among patients with and without T2DM. FOXO1 gene relative expression in blood peripheral cells is elevated in patients with NSTEMI regardless of the presence of T2DM. FOXO1 expression does not decrease after successful percutaneous coronary intervention and is not correlated with the severity of CAD in patients with NSTEMI.
众所周知，慢性高血糖或慢性炎症会导致内皮细胞中FOXO1和血管生成素-2基因（ANGPT2）表达上调。糖尿病和心肌缺血患者外周血细胞中ANGPT2和FOXO1的相对基因表达尚未得到广泛研究。
我们的目的是评估非ST段抬高型心肌梗死（NSTEMI）患者外周血细胞中ANGPT2和FOXO1基因的表达情况，这些患者包括有和没有2型糖尿病（T2DM）的， 并将其与T2DM患者和对照组的结果进行比较。
这是一项多中心前瞻性研究，纳入了138例有或无T2DM的NSTEMI患者、T2DM患者和一个对照组。测量了每位患者外周血细胞中FOXO1、ANGPT2、TBP（TATA盒结合蛋白 - 作为参照基因）的基因表达水平。进行了心电图检查和评估射血分数（EF）的超声心动图检查。NSTEMI患者接受了紧急（<24小时）冠状动脉造影，并计算了SYNTAX评分和GRACE 2.0评分。
在所分析样本的血沉棕黄层中，ANGPT2基因的相对表达非常低，仅在所有组中的11例患者中可检测到（占所有患者的8.66%）。无论是否存在T2DM，NSTEMI患者中FOXO1基因的相对表达水平（中位数相对表达 = 1.39）显著高于非NSTEMI患者（中位数 = 1.09）（ = 1578，<0.05）。FOXO1基因的相对表达与NSTEMI患者的GRACE 2.0评分或SYNTAX评分无关。成功进行血管成形术后，我们未观察到FOXO1基因表达有任何显著变化。
根据我们的结果可以得出结论，分析外周血细胞中ANGPT2基因的相对表达对评估有或无T2DM患者的CAD复杂性没有作用。无论是否存在T2DM，NSTEMI患者外周血细胞中FOXO1基因的相对表达都会升高。成功进行经皮冠状动脉介入治疗后，FOXO1表达不会降低，并且与NSTEMI患者CAD的严重程度无关。
