通过无标记梯度光干涉显微镜监测潜伏性HIV的重新激活

Monitoring reactivation of latent HIV by label-free gradient light interference microscopy.

作者信息

Goswami Neha, Lu Yiyang, Kandel Mikhail E, Fanous Michael J, Bohn-Wippert Kathrin, Tevonian Erin N, Dar Roy D, Popescu Gabriel

机构信息

Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

Quantitative Light Imaging Laboratory, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

出版信息

iScience. 2021 Aug 4;24(8):102940. doi: 10.1016/j.isci.2021.102940. eCollection 2021 Aug 20.

DOI:10.1016/j.isci.2021.102940

PMID:34430819

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8367845/

Abstract

Human immunodeficiency virus (HIV) can infect cells and take a quiescent and nonexpressive state called latency. In this study, we report insights provided by label-free, gradient light interference microscopy (GLIM) about the changes in dry mass, diameter, and dry mass density associated with infected cells that occur upon reactivation. We discovered that the mean cell dry mass and mean diameter of latently infected cells treated with reactivating drug, TNF-α, are higher for latent cells that reactivate than those of the cells that did not reactivate. Cells with mean dry mass and diameter less than approximately 10 pg and 8 μm, respectively, remain exclusively in the latent state. Also, cells with mean dry mass greater than approximately 28-30 pg and mean diameter greater than 11-12 μm have a higher probability of reactivating. This study is significant as it presents a new label-free approach to quantify latent reactivation of a virus in single cells.

摘要

人类免疫缺陷病毒（HIV）可感染细胞并进入一种称为潜伏期的静止且无表达状态。在本研究中，我们报告了无标记梯度光干涉显微镜（GLIM）所提供的关于再激活时与受感染细胞相关的干质量、直径和干质量密度变化的见解。我们发现，用再激活药物肿瘤坏死因子-α（TNF-α）处理的潜伏感染细胞，再激活的潜伏细胞的平均细胞干质量和平均直径高于未再激活的细胞。平均干质量和直径分别小于约10 pg和8 μm的细胞完全保持潜伏状态。此外，平均干质量大于约28 - 30 pg且平均直径大于11 - 12 μm的细胞具有更高的再激活概率。本研究意义重大，因为它提出了一种新的无标记方法来量化单细胞中病毒的潜伏再激活。