微卫星标记在印度小狐（Urva auropunctata）中的开发与特征分析。

Development and characterization of microsatellite markers in the small Indian mongoose (Urva auropunctata).

机构信息

Okinawa University, Naha, 902-8521, Japan.

Faculty of Law, Economics and Management, Okinawa University, Naha, 902-8521, Japan.

出版信息

Mol Biol Rep. 2021 Oct;48(10):7029-7034. doi: 10.1007/s11033-021-06655-9. Epub 2021 Aug 24.

DOI:10.1007/s11033-021-06655-9

PMID:34431036

Abstract

BACKGROUND

The small Indian mongoose (Urva auropunctata) is one of the world's worst invasive alien species and eradication programs are ongoing worldwide. The development of individual and sex identification markers will improve their management.

METHODS AND RESULTS

We searched for novel mongoose microsatellite markers using genome-wide screening and identified 115,265 tetra-nucleotide repeat loci. Of 96 loci tested, 17 were genotyped in 28 mongooses from the Okinawa population. The genetic diversity analysis showed that the average expected and observed heterozygosity and number of alleles were 0.55, 0.56, and 2.94, respectively. Of 17 loci, one deviated from Hardy-Weinberg equilibrium and six loci pairs were likely linked to each other. However, we succeed in identifying all individuals using all of the microsatellite loci. The novel sex identification markers worked successfully in a test using sex known samples.

CONCLUSION

Our novel microsatellite and sex identification markers should be useful in studies of individual identification and population genetics of the mongoose.

摘要

背景

印度小食蚁兽（Urva auropunctata）是世界上最恶劣的入侵外来物种之一，全球范围内正在开展根除计划。开发个体和性别识别标记将有助于改善对其的管理。

方法和结果

我们使用全基因组筛选技术寻找新型食蚁兽微卫星标记，并鉴定出 115265 个四核苷酸重复位点。在 96 个测试的位点中，有 17 个在来自冲绳种群的 28 只食蚁兽中进行了基因分型。遗传多样性分析表明，平均预期和观察杂合度和等位基因数分别为 0.55、0.56 和 2.94。在 17 个位点中，有一个偏离哈迪-温伯格平衡，有 6 个位点对可能彼此连锁。然而，我们成功地使用所有微卫星位点识别出了所有个体。新型性别识别标记在使用已知性别的样本进行的测试中取得了成功。