Katrenčíková Barbora, Vaváková Magdaléna, Paduchová Zuzana, Nagyová Zuzana, Garaiova Iveta, Muchová Jana, Ďuračková Zdenka, Trebatická Jana
Faculty of Medicine, Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Comenius University, Sasinkova 2, 813 72 Bratislava, Slovakia.
Juvenalia, s.r.o., Paediatric Centre, Veľkoblahovská 44A, 929 01 Dunajská Streda, Slovakia.
Antioxidants (Basel). 2021 Aug 5;10(8):1256. doi: 10.3390/antiox10081256.
Oxidative stress (OS) is thought to play a role in mental disorders. However, it is not clear whether the OS is the cause or consequence of the disorder. We investigated markers of oxidative stress (8-isoprostane (8-IsoP-U), lipoperoxides (LP), advanced oxidation protein products (AOPP) and nitrotyrosine (NT)) and antioxidant protection (Trolox equivalent antioxidant capacity (TEAC), activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) in 60 paediatric and adolescent patients with depressive disorder (DD) compared to healthy controls. The patients were divided into two groups (1:1). One group received an emulsion of omega-3 fatty acid (FA), and the other group an emulsion of sunflower oil with omega-6 FA for 12 weeks. The levels of 8-IsoP-U, AOPP and NT were increased, and GPx activity was decreased in patients compared to the controls. We found a significant positive correlation of the Children's Depression Inventory score with NT and a negative correlation with TEAC, SOD and GPx. NT correlated positively with the baseline omega-6/omega-3 FA ratio and a negatively with SOD. A supplementation with omega-3 FA, but not with omega-6 FA, decreased 8-IsoP-U, AOPP, NT levels and increased TEAC and SOD activity. Our results suggest that NT may play a role in the pathophysiology of DD, while elevated isoprostane is likely caused by the high omega-6/omega-3 FA ratio. Omega-3 FA supplementation reduces oxidative stress in patients with DD. This study was registered with the ISRCTN registry (ISRCTN81655012).
氧化应激（OS）被认为在精神障碍中起作用。然而，尚不清楚OS是该疾病的原因还是结果。我们调查了60名患有抑郁症（DD）的儿科和青少年患者与健康对照相比的氧化应激标志物（8-异前列腺素（8-IsoP-U）、脂质过氧化物（LP）、晚期氧化蛋白产物（AOPP）和硝基酪氨酸（NT））以及抗氧化保护作用（Trolox等效抗氧化能力（TEAC）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和过氧化氢酶（CAT）的活性）。患者被分为两组（1:1）。一组接受ω-3脂肪酸（FA）乳剂，另一组接受含有ω-6 FA的向日葵油乳剂，为期12周。与对照组相比，患者的8-IsoP-U、AOPP和NT水平升高，GPx活性降低。我们发现儿童抑郁量表评分与NT呈显著正相关，与TEAC、SOD和GPx呈负相关。NT与基线ω-6/ω-3 FA比值呈正相关，与SOD呈负相关。补充ω-3 FA而非ω-6 FA可降低8-IsoP-U、AOPP、NT水平，并提高TEAC和SOD活性。我们的结果表明，NT可能在DD的病理生理学中起作用，而异前列腺素升高可能是由高ω-6/ω-3 FA比值引起的。补充ω-3 FA可降低DD患者的氧化应激。本研究已在ISRCTN注册中心注册（ISRCTN81655012）。
