Výrostková Jana, Regecová Ivana, Dudriková Eva, Marcinčák Slavomír, Vargová Mária, Kováčová Mariana, Maľová Jana
Department of Food Hygiene Technology and Safety, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.
Department of Public Veterinary Medicine and Animal Welfare, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.
Foods. 2021 Aug 10;10(8):1844. doi: 10.3390/foods10081844.
This study aimed to calculate the proportion of antibiotic resistance profiles of , and isolated from traditional sheep and goat cheeses obtained from a selected border area of Slovakia with Hungary (region Slanské vrchy). A total of 110 sp. were isolated from cheese samples, of which 52 strains ( (12), (28), (12)) were represented. After isolation and identification by polymerase chain reaction and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry, the enterococci (, and ) were submitted to susceptibility tests against nine antimicrobial agents. In general, strains of were more resistant than and . A high percentage of resistance was noted in to rifampicin (100%), vancomycin (85.7%), teicoplanin (71.4%), erythromycin (71.4%), minocycline (57.1%), nitrofurantoin (57.1%), ciprofloxacin (14.3%), and levofloxacin (14.3%). showed resistance to rifampicin (100%), teicoplanin (100%), vancomycin (66.7%), erythromycin (66.7%), nitrofurantoin (66.7%), and minocycline (33.3%), and showed resistance to vancomycin, teicoplanin, and erythromycin (100%). Multidrug-resistant strains were confirmed in 80% of the 52 strains in this study. Continuous identification of sp. and monitoring of their incidence and emerging antibiotic resistance is important in order to prevent a potential risk to public health caused by the contamination of milk and other dairy products, such as cheeses, made on farm level.
本研究旨在计算从斯洛伐克与匈牙利接壤的选定地区(斯拉诺山地区)获得的传统绵羊和山羊奶酪中分离出的粪肠球菌、屎肠球菌和鸟肠球菌的抗生素耐药谱比例。从奶酪样品中共分离出110株肠球菌,其中有52株(粪肠球菌12株、屎肠球菌28株、鸟肠球菌12株)。通过聚合酶链反应和基质辅助激光解吸电离飞行时间质谱进行分离和鉴定后,对这些肠球菌(粪肠球菌、屎肠球菌和鸟肠球菌)进行了针对九种抗菌剂的药敏试验。总体而言,屎肠球菌菌株比粪肠球菌和鸟肠球菌更具耐药性。屎肠球菌对利福平(100%)、万古霉素(85.7%)、替考拉宁(71.4%)、红霉素(71.4%)、米诺环素(57.1%)、呋喃妥因(57.1%)、环丙沙星(14.3%)和左氧氟沙星(14.3%)呈现出高比例耐药。鸟肠球菌对利福平(100%)、替考拉宁(100%)、万古霉素(66.7%)、红霉素(66.7%)、呋喃妥因(66.7%)和米诺环素(33.3%)耐药,而粪肠球菌对万古霉素、替考拉宁和红霉素耐药率为100%。本研究中52株菌株中有80%被确认为多重耐药菌株。持续鉴定肠球菌菌株并监测其发生率和新出现的抗生素耐药性对于预防农场层面生产的牛奶和其他乳制品(如奶酪)污染所导致的公共卫生潜在风险至关重要。