小鼠CD8 T细胞迁移和CXCR3内化实验。
Mouse CD8 T Cell Migration and CXCR3 Internalization Assays.
作者信息
Da Silva Rosa Barreira, Albert Matthew L
机构信息
Cancer Immunology, Genentech, South San Francisco, USA.
Laboratory of Dendritic Cell Immunobiology, Institut Pasteur, Paris, France.
出版信息
Bio Protoc. 2017 Mar 20;7(6):e2185. doi: 10.21769/BioProtoc.2185.
Chemokines are molecules that regulate the positioning of cells during homeostasis and inflammation. CXCL10 is an interferon-induced chemokine that attracts cells that express the chemokine receptor CXCR3 on their surface. CXCL10 expression is often induced upon inflammation and guides lymphocytes, such as T and NK cells, into the injured tissues. Notably, CXCL10 binding to CXCR3 induces receptor internalization and, therefore, low CXCR3 levels in cells positive for CXCR3 expression can be indicative of chemokine signaling. Here, we describe an method to evaluate the ability of murine CD8 T cells to migrate towards recombinant murine CXCL10; and a flow cytometry assay to measure CXCR3 expression levels at the surface of T cells, after exposure to different doses of chemokine.
趋化因子是在稳态和炎症过程中调节细胞定位的分子。CXCL10是一种干扰素诱导的趋化因子，可吸引表面表达趋化因子受体CXCR3的细胞。CXCL10的表达通常在炎症时被诱导，并引导淋巴细胞，如T细胞和NK细胞，进入受损组织。值得注意的是，CXCL10与CXCR3的结合会诱导受体内化，因此，CXCR3表达阳性的细胞中低水平的CXCR3可指示趋化因子信号传导。在这里，我们描述了一种评估小鼠CD8 T细胞向重组小鼠CXCL10迁移能力的方法；以及一种流式细胞术检测方法，用于测量在暴露于不同剂量趋化因子后T细胞表面CXCR3的表达水平。
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