Department of Plant Biosecurity and MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, People's Republic of China.
Methods Mol Biol. 2022;2360:307-315. doi: 10.1007/978-1-0716-1633-8_22.
The application of the RNA interference (RNAi) mechanism promotes the development of novel approaches toward sustainable crop protection. Compared with traditional double-stranded (ds)RNA delivery systems, nanoparticles offer great advantages in delivering dsRNA to improve RNAi efficiency, thus promoting the development and practice of RNAi-based pest management strategies. Here, we described a transdermal dsRNA delivery system with a nanosized star polycation, and presented a method to improve RNAi efficiency to increase the control effect against aphids. Insect gene functional analysis and pest management can be achieved by this method.
RNA 干扰（RNAi）机制的应用促进了可持续作物保护的新方法的发展。与传统的双链 RNA（dsRNA）递药系统相比，纳米颗粒在递药方面具有很大的优势，可提高 RNAi 效率，从而促进 RNAi 为基础的害虫管理策略的发展和实践。在这里，我们描述了一种具有纳米级星形聚阳离子的经皮 dsRNA 递药系统，并提出了一种提高 RNAi 效率的方法，以增加对蚜虫的控制效果。该方法可用于昆虫基因功能分析和害虫防治。
