一例病因不明的急性呼吸窘迫综合征患者的SARS-CoV-2检测呈阳性回顾性分析

Positive Retrospective SARS-CoV-2 Testing in a Case of Acute Respiratory Distress Syndrome of Unknown Etiology.

作者信息

Burkett A, McElwee S, Margaroli C, Bajpai P, Elkholy A, Manne U, Wille K, Benson P

机构信息

Department of Pathology, Division of Anatomic Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA.

Department of Medicine, Division of Cardiovascular Disease, University of Alabama at Birmingham, Birmingham, Alabama, USA.

出版信息

Case Rep Pulmonol. 2021 Aug 28;2021:5484239. doi: 10.1155/2021/5484239. eCollection 2021.

DOI:10.1155/2021/5484239

PMID:34513107

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8424244/

Abstract

In order to elucidate the cause of acute respiratory distress syndrome of unknown etiology in a pre-pandemic patient, molecular techniques were used for detection of SARS-CoV-2. We used a SARS-CoV-2 nucleocapsid protein immunofluorescence stain to retrospectively identify an individual with diffuse alveolar damage on autopsy histology who had negative respiratory virus panel results in February, 2020, in Birmingham, Alabama. In situ hybridization for SARS-CoV-2 RNA revealed evidence of widespread multiorgan SARS-CoV-2 infection. This death antecedes the first reported death of a State of Alabama resident diagnosed with SARS-CoV-2 by 26 days.

摘要

为了阐明一名大流行前病因不明的急性呼吸窘迫综合征患者的病因，采用分子技术检测严重急性呼吸综合征冠状病毒2（SARS-CoV-2）。我们使用SARS-CoV-2核衣壳蛋白免疫荧光染色，对2020年2月在阿拉巴马州伯明翰进行尸检组织学检查时出现弥漫性肺泡损伤、呼吸道病毒检测结果为阴性的一名患者进行回顾性鉴定。SARS-CoV-2 RNA的原位杂交显示了多器官广泛感染SARS-CoV-2的证据。该患者死亡时间比阿拉巴马州首例确诊感染SARS-CoV-2的居民早26天。