软琼脂集落形成试验作为癌变的一个标志。
Soft Agar Colony Formation Assay as a Hallmark of Carcinogenesis.
作者信息
Du Feng, Zhao Xiaodi, Fan Daiming
机构信息
State key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, China.
出版信息
Bio Protoc. 2017 Jun 20;7(12):e2351. doi: 10.21769/BioProtoc.2351.
Abstract
Soft agar colony formation assay is established to estimate the anchorage-independent growth ability of cells. In this assay, a bottom layer of agar with complete media is poured and solidified first, followed by an upper layer containing a specified number of cells suspended in medium-agar mixture. After two weeks of incubation, the number of colonies will be counted, serving as an indicator of malignancy of tumor cells.
摘要
软琼脂集落形成试验用于评估细胞的不依赖贴壁生长能力。在此试验中，首先倒入含有完全培养基的底层琼脂并使其凝固，接着加入上层琼脂，其中含有悬浮在培养基 - 琼脂混合物中的特定数量细胞。孵育两周后，将对集落数量进行计数，作为肿瘤细胞恶性程度的指标。
相似文献
1
Soft Agar Colony Formation Assay as a Hallmark of Carcinogenesis.软琼脂集落形成试验作为癌变的一个标志。
Bio Protoc. 2017 Jun 20;7(12):e2351. doi: 10.21769/BioProtoc.2351.
2
The soft agar colony formation assay.软琼脂集落形成试验。
J Vis Exp. 2014 Oct 27(92):e51998. doi: 10.3791/51998.
3
Evaluating the Epithelial-Mesenchymal Program in Human Breast Epithelial Cells Cultured in Soft Agar Using a Novel Macromolecule Extraction Protocol.使用一种新型大分子提取方案评估在软琼脂中培养的人乳腺上皮细胞中的上皮-间质程序。
Cancers (Basel). 2021 Feb 15;13(4):807. doi: 10.3390/cancers13040807.
4
Functional differences between cells from MLR colonies grown in soft agar cultures.在软琼脂培养物中生长的混合淋巴细胞反应（MLR）集落细胞之间的功能差异。
J Immunol. 1980 Sep;125(3):1130-5.
5
Clonogenic cell assay for anchorage-dependent squamous carcinoma cell lines using limiting dilution.使用有限稀释法对贴壁依赖性鳞状癌细胞系进行克隆形成细胞测定。
Int J Cancer. 1989 Jul 15;44(1):131-6. doi: 10.1002/ijc.2910440123.
6
Dichloroacetate and trichloroacetate promote clonal expansion of anchorage-independent hepatocytes in vivo and in vitro.二氯乙酸盐和三氯乙酸盐在体内和体外均能促进非贴壁依赖性肝细胞的克隆扩增。
Toxicol Appl Pharmacol. 1998 Jun;150(2):287-94. doi: 10.1006/taap.1998.8417.
7
Colony morphology and heritability of anchorage-independent growth among spontaneously transformed Balb/3T3 cells.自发转化的Balb/3T3细胞的集落形态及非贴壁依赖性生长的遗传性
J Natl Cancer Inst. 1983 Jun;70(6):1087-96.
8
The characteristics of an anchorage-independent clonal agar assay for primary explanted bovine granulosa cells.原代培养的牛颗粒细胞非锚定依赖克隆琼脂试验的特点。
J Cell Physiol. 1982 Nov;113(2):224-30. doi: 10.1002/jcp.1041130207.
9
Soft agar colony formation of mouse epidermal cells during the early phase of two-stage chemically induced carcinogenesis.两阶段化学诱导致癌早期小鼠表皮细胞的软琼脂集落形成
Jpn J Cancer Res. 1985 May;76(5):321-3.
10
Correlation of patterns of anchorage-independent growth with in vivo behavior of cells from a murine fibrosarcoma.小鼠纤维肉瘤细胞的非锚定依赖性生长模式与体内行为的相关性。
Proc Natl Acad Sci U S A. 1980 Feb;77(2):1039-43. doi: 10.1073/pnas.77.2.1039.
引用本文的文献
1
Scaffold-Hopping Design and Synthesis of Thieno[3,2-]pyrimidines: Anticancer Activity, Apoptosis Induction, and In Silico Inhibition of CDKs.噻吩并[3,2 -]嘧啶的骨架跃迁设计与合成：抗癌活性、凋亡诱导及对细胞周期蛋白依赖性激酶的计算机模拟抑制
Int J Mol Sci. 2025 Sep 2;26(17):8528. doi: 10.3390/ijms26178528.
2
Exploring the Anticancer Activity of Leaves: Selective Effects on Triple-Negative Breast Cancer and HPV16-Positive Tumorigenic Cells.探索树叶的抗癌活性：对三阴性乳腺癌和HPV16阳性致瘤细胞的选择性作用。
Life (Basel). 2025 Jul 11;15(7):1090. doi: 10.3390/life15071090.
3
Establishment and Partial Characterization of Canine Mammary Tumor Cell Lines.犬乳腺肿瘤细胞系的建立及部分特性研究
Animals (Basel). 2025 Jul 7;15(13):1991. doi: 10.3390/ani15131991.
4
Inorganic Arsenite [As (III)] Represses Human Renal Progenitor Cell Characteristics and Induces Neoplastic-like Transformation.无机亚砷酸盐[As(III)]抑制人肾祖细胞特性并诱导肿瘤样转化。
Cells. 2025 Jun 10;14(12):877. doi: 10.3390/cells14120877.
5
A High-Throughput Neurosphere-Based Colony Formation Assay to Test Drug and Radiation Sensitivity of Different Patient-Derived Glioblastoma Lines.一种基于高通量神经球的集落形成试验，用于检测不同患者来源的胶质母细胞瘤细胞系的药物和辐射敏感性。
Cells. 2024 Dec 3;13(23):1995. doi: 10.3390/cells13231995.
6
The Abnormal Proliferation of Midbrain Dopamine Cells From Human Pluripotent Stem Cells Is Induced by Exposure to the Tumor Microenvironment.人多能干细胞来源的中脑细胞在肿瘤微环境中异常增殖。
CNS Neurosci Ther. 2024 Nov;30(11):e70117. doi: 10.1111/cns.70117.
7
Betaine Induces Apoptosis and Inhibits Invasion in OSCC Cell Lines.甜菜碱诱导口腔鳞状细胞癌细胞凋亡和抑制侵袭。
Int J Mol Sci. 2024 Sep 25;25(19):10295. doi: 10.3390/ijms251910295.
8
PPM1D activity promotes cellular transformation by preventing senescence and cell death.PPM1D 活性通过防止衰老和细胞死亡促进细胞转化。
Oncogene. 2024 Oct;43(42):3081-3093. doi: 10.1038/s41388-024-03149-3. Epub 2024 Sep 5.
9
Sulfated Polyhydroxysteroid Glycosides from the Sea of Okhotsk Starfish and Potential Mechanisms for Their Observed Anti-Cancer Activity against Several Types of Human Cancer Cells.来自鄂霍次克海海星的硫酸多羟甾苷及其对几种人类癌细胞的抗肿瘤活性的潜在机制。
Mar Drugs. 2024 Jun 26;22(7):294. doi: 10.3390/md22070294.
10
NPRL2 is required for proliferation of oncogenic Ras-transformed bronchial epithelial cells.致癌性Ras转化的支气管上皮细胞增殖需要NPRL2。
Cell Div. 2024 Jun 24;19(1):22. doi: 10.1186/s13008-024-00126-w.
本文引用的文献
1
KRAS/NF-κB/YY1/miR-489 Signaling Axis Controls Pancreatic Cancer Metastasis.KRAS/NF-κB/YY1/miR-489 信号轴调控胰腺癌转移。
Cancer Res. 2017 Jan 1;77(1):100-111. doi: 10.1158/0008-5472.CAN-16-1898. Epub 2016 Oct 28.
2
Anoikis: an emerging hallmark in health and diseases.细胞失巢凋亡：健康与疾病中的新兴标志。
J Pathol. 2012 Jan;226(2):380-93. doi: 10.1002/path.3000.
3
Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer.哺乳动物细胞的体外克隆生长；有无饲养层时单个海拉细胞形成的集落的生长特性。
J Exp Med. 1956 Feb 1;103(2):273-83. doi: 10.1084/jem.103.2.273.
4
Type beta transforming growth factor: a bifunctional regulator of cellular growth.β型转化生长因子：细胞生长的双功能调节因子。
Proc Natl Acad Sci U S A. 1985 Jan;82(1):119-23. doi: 10.1073/pnas.82.1.119.
5
Primary bioassay of human tumor stem cells.人类肿瘤干细胞的初步生物测定。
Science. 1977 Jul 29;197(4302):461-3. doi: 10.1126/science.560061.
6
Growth factors from murine sarcoma virus-transformed cells.来自鼠肉瘤病毒转化细胞的生长因子。
Proc Natl Acad Sci U S A. 1978 Aug;75(8):4001-5. doi: 10.1073/pnas.75.8.4001.
Zotero 插件