Autophosphorylation of lactate dehydrogenase.
作者信息
Yasykova M V, Ashmarina L I, Muronetz V I, Nagradova N K
机构信息
A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.
出版信息
Biochem Int. 1987 May;14(5):933-8.
PMID:3454647
Abstract
Incubation of rabbit muscle lactate dehydrogenase in the presence of Mg[alpha-32p]ATP results in the incorporation of the label into the protein. The autophosphorylation reaction is strongly pH-dependent. The maximal phosphorylation is observed at pH 6.8 with 3-4 moles of phosphate bound per mole of tetrameric enzyme. The enzyme-phosphate complex is readily hydrolyzed by hydroxylamine.
摘要
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