BMI1008 混合物对切口痛大鼠模型术后疼痛的影响。

Effects of a BMI1008 mixture on postoperative pain in a rat model of incisional pain.

机构信息

Department of Anesthesiology and Pain Medicine, College of Medicine, Chung-Ang University, Seoul, Korea.

出版信息

PLoS One. 2021 Sep 27;16(9):e0257267. doi: 10.1371/journal.pone.0257267. eCollection 2021.

DOI:10.1371/journal.pone.0257267

PMID:34570780

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8476004/

Abstract

BACKGROUND

The purpose of this study was to evaluate the analgesic effect of BMI1008 (a new drug containing lidocaine, methylene blue, dexamethasone and vitamin B complex) and to investigate the analgesic effect of lidocaine and BMI-L (other components of BMI1008 except lidocaine) at different concentrations in a rat model of incisional pain.

METHODS

Male Sprague-Dawley rats (250-300 g) were used for the incisional pain model simulating postoperative pain. After the operation, normal saline, various concentrations of BMI1008, lidocaine with a fixed concentration of BMI-L, and BMI-L with a fixed concentration of lidocaine were injected at the incision site. The preventive analgesic effect was evaluated using BMI1008 administered 30 min before and immediately after the operation. In addition, BMI1008 was compared with positive controls using intraperitoneal ketorolac 30 mg/kg and fentanyl 0.5 μg/kg. The mechanical withdrawal threshold was measured with a von Frey filament.

RESULTS

The analgesic effect according to the concentration of BMI1008, lidocaine with a fixed concentration of BMI-L, and BMI-L with a fixed concentration of lidocaine showed a concentration-dependent response and statistically significant difference among the groups (P <0.001, P <0.001, and P <0.001, respectively). The analgesic effect according to the time point of administration (before and after the operation) showed no evidence of a statistically significant difference between the groups (P = 0.170). Compared with the positive control groups, the results showed a statistically significant difference between the groups (P = 0.024).

CONCLUSION

BMI1008 showed its analgesic effect in a rat model of incisional pain in a concentration-dependent manner. Moreover, BMI-L showed an additive effect on the analgesic effect of lidocaine.

摘要

背景

本研究旨在评估 BMI1008（一种含有利多卡因、亚甲蓝、地塞米松和维生素 B 复合物的新药）的镇痛效果，并研究利多卡因和 BMI-L（BMI1008 中的其他成分）在切口痛大鼠模型中的不同浓度下的镇痛效果。

方法

雄性 Sprague-Dawley 大鼠（250-300g）用于模拟术后疼痛的切口痛模型。手术后，在切口部位注射生理盐水、不同浓度的 BMI1008、固定浓度的 BMI-L 利多卡因和固定浓度的 lidocaine 的 BMI-L。用 BMI1008 在术前 30 分钟和术后即刻给药评估预防镇痛效果。此外，用腹腔内酮咯酸 30mg/kg 和芬太尼 0.5μg/kg 与阳性对照物进行比较。用 von Frey 细丝测量机械性撤缩阈值。

结果

根据 BMI1008 的浓度、固定浓度的 BMI-L 利多卡因和固定浓度的 lidocaine 的 BMI-L 的镇痛效果呈浓度依赖性反应，各组间差异有统计学意义（P<0.001、P<0.001 和 P<0.001）。根据给药时间点（手术前后）的镇痛效果，组间无统计学差异（P=0.170）。与阳性对照组相比，组间差异有统计学意义（P=0.024）。