口咽癌和喉癌及慢性炎症患者中 HPV 的基因组和血清型。

Genome of and Serotype of HPV Detected in Oropharyngeal and Laryngeal Cancer and Chronic Inflammation Patients.

机构信息

Department of Otorhinolaryngology and Maxilofacial Surgery, Military University Hospital Prague, 16902 Prague, Czech Republic.

Third Faculty of Medicine, Charles University, 10000 Prague, Czech Republic.

出版信息

Int J Environ Res Public Health. 2021 Sep 10;18(18):9545. doi: 10.3390/ijerph18189545.

DOI:10.3390/ijerph18189545

PMID:34574466

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8470705/

Abstract

OBJECTIVE

Oropharyngeal/laryngeal carcinoma are common cancers of the upper aerodigestive system. Human papillomavirus (HPV) is described as the most frequent in the cancer of unknown primary. The presence of (HP) in the oral cavity is discussed in some papers.

THE AIM OF STUDY

To analyze the incidence of HPV and HP in oropharyngeal/laryngeal cancer persons versus persons with chronic tonsillar inflammation and healthy persons.

METHODS

The samples were taken in three groups: (1) tissue of oropharynx/larynx cancer (103 specimens); (2) tissue of palatine tonsils (85 specimens); and (3) healthy control group (50 specimens). We analyzed the presence of HP (PCR) and HPV genomic DNA (Sacace HPV High-Risk Screen Real-TM Quant) in the samples.

RESULTS

HP was detected in 86 samples (83.5%) and high-risk HPV in 62 samples (60.2%). We found a very high incidence of HP. In the cancer group, HP was detected in 82.5% cases and HPV positivity in 57.8%. In total, 7.2% of the cancer patients were negative for HP and HPV together. In turn, 53.6% of the cancer patients were positive for HP and HPV together. Four cases (4.2%) were positive for HPV only. VacA positivity was detected in 82 (79.6%) of the cancer cases and VacA negativity in 21 (20.4%) if the cancer cases. The incidence of HP in chronic inflammation ( = 85) was 65 cases (76.5%) and the incidence of HPV was 38 cases (44.7%). VacA positivity was detected in 59 (69.4%) of the chronic inflammation cases and VacA negativity was found in 26 (30.6%) of the chronic inflammation cases. Regarding the control group, we found HP positivity in 5 cases (11.1%) and HPV positivity in 19 cases (42.2%). There was VacA positivity in 6 cases (50.0%) of the control group. Statistically significantly lower prevalence of HP ( < 0.001) and HPV ( = 0.006) was found in the control group.

CONCLUSIONS

We suggest that the palatine tonsils are colonized by HP. In our study, HP was present in oropharyngeal cancer in more cases in comparison with HPV infection. The presence of VacA from HP can have an influence on the human epithelial and immune cells' regulation ways. Our results do not support idea that the CagA-positive HP is a primary carcinogen in oropharyngeal area.

摘要

目的

口咽/喉癌是上呼吸道和消化道常见的癌症。人乳头瘤病毒（HPV）是癌症中最常见的未知原发性病毒。一些文献中讨论了口腔中 HPV 的存在。

研究目的

分析 HPV 和 HP 在口咽/喉癌患者与慢性扁桃体炎患者和健康人群中的存在情况。

方法

样本取自三组：（1）口咽/喉癌组织（103 例）；（2）腭扁桃体组织（85 例）；和（3）健康对照组（50 例）。我们分析了样本中 HP（PCR）和 HPV 基因组 DNA（Sacace HPV High-Risk Screen Real-TM Quant）的存在情况。

结果

HP 在 86 例（83.5%）样本中被检测到，高危 HPV 在 62 例（60.2%）样本中被检测到。我们发现 HP 的发病率非常高。在癌症组中，HP 在 82.5%的病例中被检测到，HPV 阳性率为 57.8%。总的来说，7.2%的癌症患者同时检测到 HP 和 HPV 阴性。相反，53.6%的癌症患者同时检测到 HP 和 HPV 阳性。4 例（4.2%）仅检测到 HPV 阳性。在 82 例（79.6%）癌症病例中检测到 VacA 阳性，在 21 例（20.4%）癌症病例中检测到 VacA 阴性。慢性炎症（=85）中 HP 的发病率为 65 例（76.5%），HPV 的发病率为 38 例（44.7%）。在 59 例（69.4%）慢性炎症病例中检测到 HP 阳性，在 26 例（30.6%）慢性炎症病例中检测到 HP 阴性。在对照组中，我们发现 HP 阳性 5 例（11.1%），HPV 阳性 19 例（42.2%）。对照组中有 6 例（50.0%）检测到 VacA 阳性。对照组中 HP（<0.001）和 HPV（=0.006）的阳性率显著降低。