UHPLC Analysis of Houtt. Rhizome Preparations Regarding Stilbene and Anthranoid Composition and Their Antimycobacterial Activity Evaluation.

Houtt. is a critical invasive alien plant in Europe and North America with a drastic impact on native flora. However, has medicinal potential, especially as a source of stilbenes. In order to explore the potential of simple extractions of , we conducted qualitative and quantitative analyses of fresh rhizome infusion, decoction, and macerates with ethanol by UHPLC-DAD-ESI-MS and UHPLC-DAD, with a focus on major constituent groups of stilbenes and anthranoids. Since rhizome extracts showed antimicrobial potential in the past, we also evaluated the antimycobacterial effect of raw extracts for the first time against . Of thirty-four characterized substances, six were stilbenes and twelve anthranoids. The main constituents, four trans-stilbenes and eight anthranoids, were quantified in a validated UHPLC-DAD method. The 38% ethanol macerate showed high stilbene (155.078 mg/100 g fluid extract) and low anthranoid content (5.420 mg/100 g fluid extract), while decoction showed the highest anthranoids. Antimycobacterial testing gave good results for all macerates (MIC 256 µg/mL) and -resveratrol (64 µg/mL). Extraction and enrichment of stilbenes from fresh plant material by simple extraction methods with food-grade solvents might encourage consideration of wild harvest of rhizomes over classic means of eradication of .