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基于纳米载体的酶固定化技术在工业中的应用。

Nanocarriers-based immobilization of enzymes for industrial application.

作者信息

Thakur Kiran, Attri Chandrika, Seth Amit

机构信息

Faculty of Applied Sciences and Biotechnology, Shoolini University, Bajhol, Solan, Himachal Pradesh India.

Department of Life Sciences (Botany), Manipur University, Imphal, India.

出版信息

3 Biotech. 2021 Oct;11(10):427. doi: 10.1007/s13205-021-02953-y. Epub 2021 Sep 7.

Abstract

Nanocarriers-based immobilization strategies are a novel concept in the enhancement of enzyme stability, shelf life and efficiency. A wide range of natural and artificial supports have been assessed for their efficacy in enzyme immobilization. Nanomaterials epitomize unique and fascinating matrices for enzyme immobilization. These structures include carbon nanotubes, superparamagnetic nanoparticles and nanofibers. These nano-based supports offer stable attachment of enzymes, thus ensuring their reusability in diverse industrial applications. This review attempts to encompass recent developments in the critical role played by nanotechnology towards the improvement of the practical applicability of microbial enzymes. Nanoparticles are increasingly being used in combination with various polymers to facilitate enzyme immobilization. These endeavors are proving to be conducive for enzyme-catalyzed industrial operations. In recent years the diversity of nanomaterials has grown tremendously, thus offering endless opportunities in the form of novel combinations for various biotransformation experimentations. These nanocarriers are advantageous for both free enzymes and whole-cell immobilization, thus demonstrating to be relatively effective in several fermentation procedures.

摘要

基于纳米载体的固定化策略是提高酶稳定性、保质期和效率的一个新概念。人们已经评估了多种天然和人工载体在酶固定化方面的功效。纳米材料是酶固定化的独特且引人入胜的基质。这些结构包括碳纳米管、超顺磁性纳米颗粒和纳米纤维。这些基于纳米的载体能使酶稳定附着,从而确保其在各种工业应用中的可重复使用性。本综述试图涵盖纳米技术在提高微生物酶实际适用性方面所起关键作用的最新进展。纳米颗粒越来越多地与各种聚合物结合使用以促进酶固定化。这些努力被证明有利于酶催化的工业操作。近年来,纳米材料的多样性大幅增长,从而为各种生物转化实验提供了无数新颖组合形式的机会。这些纳米载体对游离酶和全细胞固定化都具有优势,因此在多种发酵过程中都显示出相对有效性。

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