基于半花菁骨架的用于体内检测过氧化氢的近红外荧光探针。
A near-infrared fluorescent probe based on the hemicyanine skeleton for the detection of hydrogen peroxide in vivo.
机构信息
Institute of Optical Functional Materials for Biomedical Imaging, School of Chemistry and Pharmaceutical Engineering, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, Shandong 271016, PR China.
Institute of Optical Functional Materials for Biomedical Imaging, School of Chemistry and Pharmaceutical Engineering, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, Shandong 271016, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Feb 5;266:120435. doi: 10.1016/j.saa.2021.120435. Epub 2021 Sep 27.
As a member of the reactive oxygen species, hydrogen peroxide (HO) plays critical roles in oxidative stress and cell signaling. Intracellular abnormal levels of HO production are closely related to many diseases. Therefore, the real-time monitoring of HO in the cells is important. In this work, we designed a novel fluorescent probe (Mito-HO) for the specific detection of HO based on the hemicyanine skeleton, with bright near-infrared fluorescence emission. Mito-HO displayed fast response, excellent water-solubility and great fluorescence intensity enhancement after the addition of HO. Furthermore, Mito-HO has been successfully applied to image both of the exogenous and endogenous HO in cells and mice with negligible cytotoxity.
作为活性氧（ROS）的一种，过氧化氢（HO）在氧化应激和细胞信号转导中起着关键作用。细胞内 HO 产生水平异常与许多疾病密切相关。因此，实时监测细胞中的 HO 非常重要。在这项工作中，我们基于半花菁骨架设计了一种新型的荧光探针（Mito-HO），用于 HO 的特异性检测，具有明亮的近红外荧光发射。Mito-HO 具有快速的响应速度、优异的水溶性和对 HO 加入后的强荧光强度增强。此外，Mito-HO 已成功应用于细胞和小鼠中外源和内源性 HO 的成像，具有可忽略的细胞毒性。
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