Olson M V, Dutchik J E, Graham M Y, Brodeur G M, Helms C, Frank M, MacCollin M, Scheinman R, Frank T
Proc Natl Acad Sci U S A. 1986 Oct;83(20):7826-30. doi: 10.1073/pnas.83.20.7826.
An approach to global restriction mapping is described that is applicable to any complex source DNA. By analyzing a single restriction digest for each member of a redundant set of lambda clones, a data base is constructed that contains fragment-size lists for all the clones. The clones are then grouped into subsets, each member of which is related to at least one other member by a significant overlap. Finally, a tree-searching algorithm seeks restriction maps that are consistent with the fragment-size lists for all the clones in each subset. The feasibility of the approach has been demonstrated by collecting data on 5000 lambda clones containing random 15-kilobase inserts of yeast DNA. It is shown that these data can be analyzed to produce regional maps of the yeast genome, extending in some cases for over 100 kilobases. In combination with hybridization probes to previously cloned genes, these local maps are already useful for defining the physical arrangement of closely linked genes. They may in the future serve as building blocks for the construction of a continuous global map.
本文描述了一种适用于任何复杂来源DNA的全局限制酶切图谱绘制方法。通过分析一组冗余λ克隆中每个成员的单个限制酶切消化产物，构建一个数据库，其中包含所有克隆的片段大小列表。然后将克隆分组为子集，每个子集的每个成员都与至少一个其他成员有显著重叠。最后，一种树形搜索算法寻找与每个子集中所有克隆的片段大小列表一致的限制酶切图谱。通过收集关于5000个含有酵母DNA随机15千碱基插入片段的λ克隆的数据，证明了该方法的可行性。结果表明，这些数据可以进行分析以生成酵母基因组的区域图谱，在某些情况下延伸超过100千碱基。与针对先前克隆基因的杂交探针相结合，这些局部图谱已经可用于确定紧密连锁基因的物理排列。它们未来可能作为构建连续全局图谱的基础。
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