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环境条件对从发酵肉制品中分离出的乳酸菌拮抗活性的影响

The Influence of Environmental Conditions on the Antagonistic Activity of Lactic Acid Bacteria Isolated from Fermented Meat Products.

作者信息

Łepecka Anna, Szymański Piotr, Rutkowska Sylwia, Iwanowska Kinga, Kołożyn-Krajewska Danuta

机构信息

Department of Meat and Fat Technology, Prof. Waclaw Dabrowski Institute of Agriculture and Food Biotechnology-State Research Institute, Rakowiecka 36 St., 02-532 Warsaw, Poland.

Department of Food Gastronomy and Food Hygiene, Institute of Human Nutrition Sciences, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159c St., 02-776 Warsaw, Poland.

出版信息

Foods. 2021 Sep 25;10(10):2267. doi: 10.3390/foods10102267.

Abstract

The aim of this study was to determine the impact of environmental conditions on the antimicrobial properties of 21 lactic acid bacteria strains in the selected indicator bacteria. To assess the antimicrobial activity of the whole bacteria culture (WBC), the agar well diffusion method was used. The interference of LAB strains with the growth of the selected indicator bacteria was evaluated by incubating co-cultures in the food matrix. Based on the conducted research, it was found that environmental conditions have a significant impact on the antimicrobial activity of lactic acid bacteria strains. The highest antimicrobial activity was recorded under optimal conditions for the development of LAB, the incubation time being different depending on the indicator strain used. The tested LAB strains were characterized by a high ability to inhibit indicator strains, especially in the food matrix. These results led us to further characterize and purify the antimicrobial compound produced by lactic acid bacteria taking into account changing environmental conditions.

摘要

本研究的目的是确定环境条件对所选指示菌中21株乳酸菌菌株抗菌特性的影响。为评估全菌培养物(WBC)的抗菌活性,采用了琼脂扩散法。通过在食品基质中共培养来评估乳酸菌菌株对所选指示菌生长的干扰。基于所进行的研究,发现环境条件对乳酸菌菌株的抗菌活性有显著影响。在乳酸菌生长的最佳条件下记录到最高的抗菌活性,孵育时间因所用指示菌株而异。所测试的乳酸菌菌株具有很高的抑制指示菌株的能力,尤其是在食品基质中。这些结果促使我们考虑不断变化的环境条件,进一步表征和纯化乳酸菌产生的抗菌化合物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b96b/8534964/bb7707f13933/foods-10-02267-g001.jpg

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