Institute of Plant Genetics, Breeding and Biotechnology, University of Life Sciences in Lublin, Akademicka 13 St., 20-950 Lublin, Poland.
Diversity Arrays Technology Pty Ltd., University of Canberra, Canberra, Kirinari St., Bruce, ACT 2617, Australia.
Int J Mol Sci. 2021 Oct 17;22(20):11183. doi: 10.3390/ijms222011183.
Crown rust, caused by f. sp. , is one of the most destructive fungal diseases of oat worldwide. Growing disease-resistant oat cultivars is the preferred method of preventing the spread of rust and potential epidemics. The object of the study was , a race-specific seedling crown rust resistant gene, highly effective at all growth stages, selected from the differential line Pc50 ( L. CW 486-1 × Pendek). A comparison of crown rust reaction as well as an allelism test showed the distinctiveness of , whereas the proportions of phenotypes in segregating populations derived from a cross with two crown rust-susceptible Polish oat cultivars, Kasztan × Pc50-5 and Bingo × Pc50-5, confirmed monogenic inheritance of the gene, indicating its usefulness in oat breeding programs. Effective gene introgression depends on reliable gene identification in the early stages of plant development; thus, the aim of the study was to develop molecular markers that are tightly linked to . Segregating populations of Kasztan × Pc50-5 were genotyped using DArT technology based on next-generation Illumina short-read sequencing. Markers associated with were located on chromosome 6A of the current version of the oat reference genome ( OT3098 v2, PepsiCo) in the region between 434,234,214 and 440,149,046 bp and subsequently converted to PCR-based SCAR (sequence-characterized amplified region) markers. Furthermore, 5426978_SCAR and 24031809_SCAR co-segregated with the resistance allele and were mapped to the partial linkage group at 0.6 and 4.0 cM, respectively. The co-dominant 58163643_SCAR marker was the best diagnostic and it was located closest to at 0.1 cM. The newly discovered, very strong monogenic crown rust resistance may be useful for oat improvement. DArT sequences converted into specific PCR markers will be a valuable tool for marker-assisted selection in breeding programs.
冠锈病，由 f. sp. 引起，是世界范围内燕麦最具破坏性的真菌病害之一。种植抗锈病燕麦品种是防止锈病传播和潜在流行的首选方法。本研究的对象是从 Pc50 差异系（ L. CW 486-1 × Pendek）中筛选出的一个在所有生长阶段都高度有效的、针对特定小种的幼苗冠锈病抗性基因 。对冠锈病反应的比较和等位基因测试表明了 的独特性，而来自与两个波兰燕麦感病品种（Kasztan × Pc50-5 和 Bingo × Pc50-5）杂交的分离群体的表型比例证实了该基因的单基因遗传，表明其在燕麦育种计划中的有用性。有效基因导入取决于在植物发育的早期阶段对基因进行可靠鉴定；因此，本研究的目的是开发与 紧密连锁的分子标记。利用基于下一代 Illumina 短读测序的 DArT 技术对 Kasztan × Pc50-5 分离群体进行了基因型分析。与 相关的标记位于当前燕麦参考基因组（PepsiCo 的 OT3098 v2）6A 染色体上，位于 434,234,214 和 440,149,046 bp 之间的区域，并随后转化为基于 PCR 的 SCAR（序列特征扩增区）标记。此外，5426978_SCAR 和 24031809_SCAR 与 抗性等位基因共分离，分别被定位在 0.6 和 4.0 cM 的部分连锁群上。共显性的 58163643_SCAR 标记是最好的诊断标记，它与 距离最近，为 0.1 cM。新发现的、非常强的单基因冠锈病抗性可能对燕麦改良有用。转化为特定 PCR 标记的 DArT 序列将成为育种计划中标记辅助选择的宝贵工具。
