肿瘤坏死因子-α -308G>A 和白细胞介素 10-1082A>G 多态性似乎是慢性 HCV 感染的预测生物标志物。
TNFA -308G>A and IL10 -1082A>G polymorphisms seem to be predictive biomarkers of chronic HCV infection.
机构信息
Laboratory of Virology, Institute of Biological Sciences, Federal University of Pará (Universidade Federal Do Pará - UFPA), Belém, Pará, Brazil.
Graduate Program in Virology, Evandro Chagas Institute/SVS/MS, Ananindeua, Pará, Brazil.
出版信息
BMC Infect Dis. 2021 Nov 3;21(1):1133. doi: 10.1186/s12879-021-06835-9.
BACKGROUND
Genetic changes may induce dysregulated cytokine production and affect the progression of the chronic disease caused by the hepacivirus C (HCV) because the balance of pro- and anti-inflammatory cytokines determines the outcome of infection. This study evaluated the TNFA -308G>A and IL10 -1082A>G polymorphisms in the susceptibility and progress of chronic hepatitis C.
METHOD
The study included 101 samples from patients with chronic hepatitis C and 300 samples from healthy donors. Polymorphisms were typed by real-time PCR and were analyzed for associations with histopathological parameters (according to METAVIR classification) and HCV viral load.
RESULTS
The polymorphic genotype for the TNFA -308G>A variant was not present in the group of patients with chronic hepatitis C and its absence could be associated with protection against HCV infection (p = 0.0477). Patients with the polymorphic genotype of the IL10 -1082A>G polymorphism had higher HCV viral load than wild-type patients (p = 0.0428). Neither polymorphism was associated with different levels of necroinflammatory activity or fibrosis scores.
CONCLUSION
Our results suggest the polymorphic genotype at TNFA -308G>A as protective against chronic HCV infection, and the polymorphic genotype at the IL10 -1082A>G variant associated with higher HCV viral load. Further studies must be performed in order to confirm these associations.
背景
遗传变化可能导致细胞因子产生失调，并影响由丙型肝炎病毒（HCV）引起的慢性疾病的进展，因为促炎和抗炎细胞因子的平衡决定了感染的结果。本研究评估了 TNFa-308G>A 和 IL10-1082A>G 多态性与慢性丙型肝炎的易感性和进展的关系。
方法
本研究包括 101 例慢性丙型肝炎患者和 300 例健康供者的样本。通过实时 PCR 对多态性进行分型，并分析其与组织病理学参数（根据 METAVIR 分类）和 HCV 病毒载量的关系。
结果
TNFa-308G>A 变异的多态基因型在慢性丙型肝炎患者中不存在，其缺失可能与对 HCV 感染的保护有关（p=0.0477）。IL10-1082A>G 多态性的患者的 HCV 病毒载量高于野生型患者（p=0.0428）。两种多态性均与不同水平的坏死性炎症活动或纤维化评分无关。
结论
我们的结果表明，TNFa-308G>A 的多态基因型对慢性 HCV 感染具有保护作用，而 IL10-1082A>G 变异的多态基因型与 HCV 病毒载量较高有关。需要进一步的研究来证实这些关联。
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