槲皮素与2-甲氧基雌二醇联合通过Wnt信号通路抑制PC-3细胞系的上皮-间质转化。
Combination of quercetin and 2-methoxyestradiol inhibits epithelial-mesenchymal transition in PC-3 cell line via Wnt signaling pathway.
作者信息
Sharma Neeti, Raut Piyush W, Baruah Meghna M, Sharma Akshay
机构信息
School of Engineering, Ajeenkya DY Patil University, Charholi Budruk, Pune, 412105, India.
Symbiosis School of Biological Sciences, Symbiosis International (Deemed University), Gram - Lavale; Taluka - Mulshi, Pune, India.
出版信息
Future Sci OA. 2021 Sep 24;7(9):FSO747. doi: 10.2144/fsoa-2021-0028. eCollection 2021 Oct.
AIM
We have previously reported that quercetin (Qu) regulates epithelial-mesenchymal transition (EMT) by modulating Wnt signaling components. In this study, we investigated the synergistic effect of Qu and 2-methoxyestradiol (2-ME) and the role of Wnt signaling components in regulating EMT in PC-3 cells.
MATERIALS & METHODS: EMT was induced by treating PC-3 cells with TGF-β, followed by evaluation of expression of EMT markers and Wnt signaling proteins in naive, induced and after exposing induced cells to Qu and 2-ME at both gene and protein level by real-time PCR (RT-PCR) and western blot, respectively.
RESULTS
Qu and 2-ME synergistically downregulated mesenchymal markers with simultaneous upregulation of epithelial markers. Wnt signaling proteins expression was also downregulated by Qu and 2-ME in TGF-β-induced EMT in PC-3 cells.
CONCLUSION
Thus, combination therapy of Qu and 2-ME could be a new promising therapeutic approach for the treatment of prostate cancer.
目的
我们之前报道过槲皮素（Qu）通过调节Wnt信号通路成分来调控上皮-间质转化（EMT）。在本研究中，我们探究了Qu与2-甲氧基雌二醇（2-ME）的协同作用以及Wnt信号通路成分在调控PC-3细胞EMT中的作用。
材料与方法
用转化生长因子-β（TGF-β）处理PC-3细胞以诱导EMT，随后分别通过实时聚合酶链反应（RT-PCR）和蛋白质免疫印迹法在基因和蛋白质水平评估未处理、诱导处理以及将诱导后的细胞暴露于Qu和2-ME后的EMT标志物和Wnt信号蛋白的表达。
结果
Qu和2-ME协同下调间充质标志物，同时上调上皮标志物。在PC-3细胞中，TGF-β诱导的EMT过程中，Qu和2-ME也下调了Wnt信号蛋白的表达。
结论
因此，Qu和2-ME联合治疗可能是一种治疗前列腺癌的新的有前景的治疗方法。
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