用于简化体内归巢肽鉴定的新工具。

New Tools for Streamlined In Vivo Homing Peptide Identification.

机构信息

Laboratory of Precision and Nanomedicine, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.

Faculty of Medicine, University of Latvia, Riga, Latvia.

出版信息

Methods Mol Biol. 2022;2383:385-412. doi: 10.1007/978-1-0716-1752-6_25.

DOI:10.1007/978-1-0716-1752-6_25

PMID:34766303

Abstract

In vivo peptide-phage display is an unbiased technique for mapping of the vascular diversity and identification of homing peptides. This chapter is intended to serve as a structured practical guide to execute in vivo T7 phage biopanning and data analysis experiments. We discuss experimental designs and protocols with emphasis on application of high-throughput sequencing-based technologies for streamlined in vivo biopanning and validation of homing peptides.

摘要

体内肽噬菌体展示是一种用于绘制血管多样性和鉴定归巢肽的无偏技术。本章旨在作为执行体内 T7 噬菌体生物淘选和数据分析实验的结构化实用指南。我们讨论了实验设计和方案，重点是应用高通量测序技术进行简化的体内生物淘选和归巢肽的验证。