Antilymphoma Effect of Incomptine A: In Vivo, In Silico, and Toxicological Studies.

Incomptine A () is a sesquiterpene lactone isolated from that induces apoptosis, reactive oxygen species production, and a differential protein expression on the U-937 (diffuse histiocytic lymphoma) cell line. In this work, the antitumor potential of was investigated on Balb/c mice inoculated with U-937 cells and through the brine shrimp lethality (BSL) test. Furthermore, was subjected to molecular docking study using as targets proteins associated with processes of cancer as apoptosis, oxidative stress, and glycolytic metabolism. In addition to determining the potential toxicity of in human, its acute toxicity was performed in mice. Results reveals that showed high antilymphoma activity and BSL with an EC of 2.4 mg/kg and LC 16.7 µg/mL, respectively. The molecular docking study revealed that has strong interaction on all targets used. In the acute oral toxicity, had a LD of 149 mg/kg. The results showed that the activities of including antilymphoma activity, BSL, acute toxicity, and in silico interactions were close to the methotrexate, an anticancer drug used as positive control. These findings suggest that may serve as a candidate for the development of a new drug to combat lymphoma.