Research Group for Food Biotechnology, Institute of Food Science and Technology, Hungarian University of Agriculture and Life Sciences, Budapest, Hungary.
BioFil Microbiological, Biotechnological and Biochemical Ltd., Budapest, Hungary.
Int J Syst Evol Microbiol. 2021 Nov;71(11). doi: 10.1099/ijsem.0.005105.
A Gram-reaction-negative bacterial strain, designated Kb22, was isolated from agricultural soil and characterized using a polyphasic approach to determine its taxonomic position. On the basis of 16S rRNA gene sequence analysis, the strain shows highest similarity (94.39 %) to M-SX103. The highest average nucleotide identity value (71.83 %) was found with T5-12, and the highest amino acid identity value (66.65 %) was found with HAL-9. Cells are aerobic, non-motile rods. The isolate was found to be positive for catalase and oxidase tests. The assembled genome of strain Kb22 has a total length of 4,06 Mb, the DNA G+C content is 38.1 mol%. The only isoprenoid quinone is menaquinone 7 (MK-7). The major fatty acids are iso-C (28.4%), summed feature 3 (C ω7 and/or iso-C 2-OH) (25.7 %) and iso-C 3-OH (19.7 %). Based on phenotypic characteristics and phylogenetic results, it is concluded that strain Kb22 is a member of the genus and represents a novel species for which the name sp. nov. is proposed. The type strain of the species is strain Kb22 (=LMG 31574=NCAIM B.02638).
一株革兰氏反应阴性的细菌菌株,被命名为 Kb22,从农业土壤中分离出来,并通过多相方法进行了特征描述,以确定其分类地位。基于 16S rRNA 基因序列分析,该菌株与 M-SX103 表现出最高的相似性(94.39%)。与 T5-12 的最高平均核苷酸同一性值(71.83%),与 HAL-9 的最高氨基酸同一性值(66.65%)。细胞是需氧的,非运动的棒状。该分离株被发现对过氧化氢酶和氧化酶测试呈阳性。Kb22 菌株的全基因组组装长度为 4.06 Mb,DNA G+C 含量为 38.1 mol%。唯一的类异戊二烯醌是甲萘醌 7(MK-7)。主要脂肪酸为异 C(28.4%)、总和特征 3(C ω7 和/或异 C 2-OH)(25.7%)和异 C 3-OH(19.7%)。基于表型特征和系统发育结果,结论是菌株 Kb22 是属的成员,代表了一个新的种,建议命名为 sp. nov.。该种的模式菌株是 Kb22 菌株(=LMG 31574=NCAIM B.02638)。