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新种,属边缘的新型物种。

sp. nov. a novel species on the borderline of the genus .

机构信息

Research Group for Food Biotechnology, Institute of Food Science and Technology, Hungarian University of Agriculture and Life Sciences, Budapest, Hungary.

BioFil Microbiological, Biotechnological and Biochemical Ltd., Budapest, Hungary.

出版信息

Int J Syst Evol Microbiol. 2021 Nov;71(11). doi: 10.1099/ijsem.0.005105.

Abstract

A Gram-reaction-negative bacterial strain, designated Kb22, was isolated from agricultural soil and characterized using a polyphasic approach to determine its taxonomic position. On the basis of 16S rRNA gene sequence analysis, the strain shows highest similarity (94.39 %) to M-SX103. The highest average nucleotide identity value (71.83 %) was found with T5-12, and the highest amino acid identity value (66.65 %) was found with HAL-9. Cells are aerobic, non-motile rods. The isolate was found to be positive for catalase and oxidase tests. The assembled genome of strain Kb22 has a total length of 4,06 Mb, the DNA G+C content is 38.1 mol%. The only isoprenoid quinone is menaquinone 7 (MK-7). The major fatty acids are iso-C (28.4%), summed feature 3 (C ω7 and/or iso-C 2-OH) (25.7 %) and iso-C 3-OH (19.7 %). Based on phenotypic characteristics and phylogenetic results, it is concluded that strain Kb22 is a member of the genus and represents a novel species for which the name sp. nov. is proposed. The type strain of the species is strain Kb22 (=LMG 31574=NCAIM B.02638).

摘要

一株革兰氏反应阴性的细菌菌株,被命名为 Kb22,从农业土壤中分离出来,并通过多相方法进行了特征描述,以确定其分类地位。基于 16S rRNA 基因序列分析,该菌株与 M-SX103 表现出最高的相似性(94.39%)。与 T5-12 的最高平均核苷酸同一性值(71.83%),与 HAL-9 的最高氨基酸同一性值(66.65%)。细胞是需氧的,非运动的棒状。该分离株被发现对过氧化氢酶和氧化酶测试呈阳性。Kb22 菌株的全基因组组装长度为 4.06 Mb,DNA G+C 含量为 38.1 mol%。唯一的类异戊二烯醌是甲萘醌 7(MK-7)。主要脂肪酸为异 C(28.4%)、总和特征 3(C ω7 和/或异 C 2-OH)(25.7%)和异 C 3-OH(19.7%)。基于表型特征和系统发育结果,结论是菌株 Kb22 是属的成员,代表了一个新的种,建议命名为 sp. nov.。该种的模式菌株是 Kb22 菌株(=LMG 31574=NCAIM B.02638)。

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