生产、分离和鉴定源自拟杆菌属的工程化细菌细胞外膜囊泡及其在疫苗开发中的应用
Production, Isolation, and Characterization of Bioengineered Bacterial Extracellular Membrane Vesicles Derived from Bacteroides thetaiotaomicron and Their Use in Vaccine Development.
机构信息
Quadram Institute, Norwich, UK.
出版信息
Methods Mol Biol. 2022;2414:171-190. doi: 10.1007/978-1-0716-1900-1_11.
PMID:34784038
Abstract
Bacterial extracellular vesicles (BEVs) possess features that make them well suited for the delivery of therapeutics and vaccines. This chapter describes methods for engineering the commensal human intestinal bacterium Bacteroides thetaiotaomicron (Bt) to produce BEVs carrying vaccine antigens and accompanying methods for isolating and purifying BEVs for mucosal vaccination regimens.
摘要
细菌细胞外囊泡（BEVs）具有使其非常适合用于递送治疗剂和疫苗的特征。本章描述了工程化共生人类肠道细菌拟杆菌（Bacteroides thetaiotaomicron，Bt）以产生携带疫苗抗原的 BEVs 的方法，以及用于分离和纯化 BEVs 以用于黏膜疫苗方案的方法。
Zotero 插件