State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Tianjin Central Hospital of Gynecology Obstetrics/Tianjin Key Laboratory of Human Development and Reproductive Regulation, Nankai University, Tianjin 300350, China.
Department of Obstetrics, Tianjin First Central Hospital, Nankai University, Tianjin 300192, China.
STAR Protoc. 2021 Nov 5;2(4):100881. doi: 10.1016/j.xpro.2021.100881. eCollection 2021 Dec 17.
Haploid trophoblast stem cells (TSCs) are advanced in studying placental development for their placental precursor and homozygous features. Here, we describe how to generate haploid-induced TSCs (haiTSCs) from haploid embryonic stem cells with a Tet-on system. Our haiTSCs can maintain haploidy long-term and can produce genome-wide mutants combined with transposons. It is promising in high-throughput genetic screening of trophoblast-specific modulators. For complete details on the use and execution of this protocol, please refer to Peng et al. (2019).
单倍体滋养层干细胞（TSCs）因其胎盘前体和纯合子特征，在研究胎盘发育方面具有优势。在这里，我们描述了如何使用 Tet-on 系统从单倍体胚胎干细胞中产生单倍体诱导的 TSCs（haiTSCs）。我们的 haiTSCs 可以长期维持单倍体，并可以与转座子结合产生全基因组突变体。它在高通量筛选滋养层特异性调节剂方面具有很大的应用前景。如需了解本方案的详细使用和操作方法，请参考 Peng 等人（2019 年）的文献。
