Department of Biochemistry, Faculty of Pharmacy, Erciyes University, Kayseri 38039, Turkey; Pharmacy Services Program, Vocational School of Health Services, Hitit University, 19000, Corum, Turkey.
Recep Tayyip Erdogan University, Faculty of Fisheries and Aquatic Sciences, 53100 Rize, Turkey.
J Biotechnol. 2022 Jan 10;343:96-101. doi: 10.1016/j.jbiotec.2021.11.009. Epub 2021 Nov 30.
Herein, we report the synthesis of taurine incorporated (sulfur containing organic molecule derived from methionine and cysteine) hybrid nanoflowers (thNFs) with an intrinsic peroxidase-mimic and antimicrobial activities in the presence of HO. Formation of thNFs using non-enzyme molecules was for the first time and systematically studied as a function of the taurine concentration, types of metal ions (Cu, Fe and Fe) and pH values of reaction solution. The peroxidase like activities of thNFs rely on Fenton-like reaction against guaiacol used as a model substrate. The efficiency of Fenton reaction can be attributed to porous structure and presence of ions of transition elements in the thNFs. The thNFs were further characterized using FTIR, XRD, SEM and EDX. The thNFs also showed remarkable antimicrobial properties against S. aureus, E. coli, B. cereus and C. albicans. We claim that nonprotein-based NFs can be considered as new generation nano-biocatalysts as an alternative to enzymes and can be used in various medicinal, biochemical, immunological, biotechnological, and industrial applications.
在此,我们报告了在 HO 存在下,通过内源性过氧化物酶模拟物和抗菌活性合成的牛磺酸(含硫有机分子,来源于蛋氨酸和半胱氨酸)混合纳米花(thNFs)。首次使用非酶分子并系统地研究了 thNFs 的形成,作为牛磺酸浓度、金属离子类型(Cu、Fe 和 Fe)和反应溶液 pH 值的函数。thNFs 的过氧化物酶样活性依赖于 Fenton 样反应,针对愈创木酚(用作模型底物)。Fenton 反应的效率可归因于 thNFs 中的多孔结构和过渡元素离子的存在。thNFs 还使用 FTIR、XRD、SEM 和 EDX 进行了进一步表征。thNFs 还对金黄色葡萄球菌、大肠杆菌、蜡状芽孢杆菌和白色念珠菌表现出显著的抗菌性能。我们声称,基于非蛋白质的 NF 可以被认为是替代酶的新一代纳米生物催化剂,并可用于各种医学、生化、免疫、生物技术和工业应用。