一种用于检测细胞外 L-乳酸的基因编码荧光生物传感器。
A genetically encoded fluorescent biosensor for extracellular L-lactate.
机构信息
Department of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, 113-0033, Japan.
Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, T2N 4N1, Canada.
出版信息
Nat Commun. 2021 Dec 6;12(1):7058. doi: 10.1038/s41467-021-27332-2.
L-Lactate, traditionally considered a metabolic waste product, is increasingly recognized as an important intercellular energy currency in mammals. To enable investigations of the emerging roles of intercellular shuttling of L-lactate, we now report an intensiometric green fluorescent genetically encoded biosensor for extracellular L-lactate. This biosensor, designated eLACCO1.1, enables cellular resolution imaging of extracellular L-lactate in cultured mammalian cells and brain tissue.
L-乳酸，传统上被认为是一种代谢废物，现在越来越被认为是哺乳动物中一种重要的细胞间能量货币。为了能够研究细胞间 L-乳酸穿梭的新兴作用，我们现在报告了一种强度计型绿色荧光遗传编码的细胞外 L-乳酸生物传感器。这个生物传感器，被指定为 eLACCO1.1，能够在培养的哺乳动物细胞和脑组织中进行细胞分辨率的细胞外 L-乳酸成像。
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